FN Archimer Export Format PT J TI Desulfobotulus mexicanus sp. nov., a novel sulfate-reducing bacterium isolated from the sediment of an alkaline crater lake in Mexico BT AF Pérez-Bernal, Maria Fernanda Brito, Elcia M. S. Bartoli, Manon Aubé, Johanne Ollivier, Bernard Guyoneaud, Rémy Hirschler-Réa, Agnès AS 1:1,2,3;2:1,2;3:1;4:3;5:1;6:3;7:1; FF 1:;2:;3:;4:;5:;6:;7:; C1 Aix Marseille Univ., Université de Toulon, CNRS, IRD, MIO UM 110, 13288, Marseille, France Laboratory of Sanitary and Environmental Engineering, Engineering Division, Campus de Guanajuato, University of Guanajuato, Guanajuato, Mexico Environmental Microbiology group, IPREM UMR CNRS 5254, Université de Pau et des Pays de l'Adour, IBEAS, Pau, France C2 UNIV AIX MARSEILLE, FRANCE UNIV GUANAJUATO, MEXICO UNIV PAU & PAYS ADOUR, FRANCE IF 2.747 TC 5 UR https://archimer.ifremer.fr/doc/00622/73396/72613.pdf LA English DT Article DE ;alkaliphilic anaerobic bacterium;sulfate reduction;Desulfobotulus AB A novel Gram-negative, non-spore-forming, vibrio-shaped, anaerobic, alkaliphilic, sulfate-reducing bacterium, designated strain PAR22NT, was isolated from sediment samples collected at an alkaline crater lake in Guanajuato (Mexico). Strain PAR22NT grew at temperatures between 15 and 37 °C (optimum, 32 °C), at pH between pH 8.3 and 10.1 (optimum, pH 9.0–9.6), and in the presence of NaCl up to 10 %. Pyruvate, 2-methylbutyrate and fatty acids (4–18 carbon atoms) were used as electron donors in the presence of sulfate as a terminal electron acceptor and were incompletely oxidized to acetate and CO2. Besides sulfate, both sulfite and elemental sulfur were also used as terminal electron acceptors and were reduced to sulfide. The predominant fatty acids were summed feature 10 (C18 : 1 ω7c and/or C18 : 1 ω9t and/or C18 : 1 ω12t), C18 : 1 ω9c and C16 : 0. The genome size of strain PAR22NT was 3.8 Mb including 3391 predicted genes. The genomic DNA G+C content was 49.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that it belongs to the genus Desulfobotulus within the class Deltaproteobacteria. Its closest phylogenetic relatives are Desulfobotulus alkaliphilus (98.4 % similarity) and Desulfobotulus sapovorans (97.9 % similarity). Based on phylogenetic, phenotypic and chemotaxonomic characteristics, we propose that the isolate represents a novel species of the genus Desulfobotulus with the name Desulfobotulus mexicanus sp. nov. The type strain is PAR22NT (=DSM 105758T=JCM 32146T). PY 2020 SO International Journal Of Systematic And Evolutionary Microbiology SN 1466-5026 PU Microbiology Society VL 70 IS 5 UT 000537392000033 BP 3219 EP 3225 DI 10.1099/ijsem.0.004159 ID 73396 ER EF