FN Archimer Export Format
PT J
TI Towards a rigorous species delimitation framework for scleractinian corals based on RAD sequencing: the case study of Leptastrea from the Indo-Pacific
BT 
AF Arrigoni, Roberto
   Berumen, Michael L.
   Mariappan, Kiruthiga G.
   Beck, Pieter S. A.
   Hulver, Ann Marie
   Montano, Simone
   Pichon, Michel
   Strona, Giovanni
   Terraneo, Tullia Isotta
   Benzoni, Francesca
AS 1:1,2,3;2:2;3:2;4:3;5:2;6:4,5;7:6;8:7;9:2,8;10:2;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;
C1 Department of Biology and Evolution of Marine Organisms (BEOM), Stazione Zoologica Anton Dohrn Napoli, Villa Comunale, 80121, Naples, Italy
   Red Sea Research Center, Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia
   European Commission, Joint Research Centre (JRC), Ispra, Italy
   Dipartimento Di Scienze Dell’Ambiente E del Territorio, Università Degli Studi Di Milano-Bicocca, Piazza della Scienza 1, 20126, Milan, Italy
   Marine Research and High Education Center, Magoodhoo Island, Faafu Atoll, Maldives
   Queensland Museum, Biodiversity and Geosciences, Townsville, QLD, 4810, Australia
   Research Centre for Ecological Change, University of Finland, 00014, Helsinki, Finland
   ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, QLD, 4810, Australia
C2 STAZ ZOOL ANTON DOHRN, ITALY
   UNIV KING ABDULLAH, SAUDI ARABIA
   JRC, ITALY
   UNIV MILANO BICOCCA, ITALY
   MARHE, MALDIVES
   QUEENSLAND MUSEUM, AUSTRALIA
   UNIV HELSINKI, FINLAND
   UNIV JAMES COOK, AUSTRALIA
IF 3.902
TC 35
UR https://archimer.ifremer.fr/doc/00623/73501/73081.pdf
LA English
DT Article
CR BIBELOT
   CORALCAL-1
   CORALCAL-2
   CORALCAL-3
   CORALCAL-4
   CORALCAL-5
   KAVIENG
   MAD
   MADANG 2012
BO Alis
   Antea
DE ;ezRAD;dDocent;Holobiont;SNAPP;Bayes factor delimitation;Morphometrics;New species
AB Accurate delimitation of species and their relationships is a fundamental issue in evolutionary biology and taxonomy and provides essential implications for conservation management. Scleractinian corals are difficult to identify because of their ecophenotypic and geographic variation and their morphological plasticity. Furthermore, phylogenies based on traditional loci are often unresolved at the species level because of uninformative loci. Here, we attempted to resolve these issues and proposed a consistent species definition method for corals by applying the genome-wide technique Restriction-site Associated DNA sequencing (RADseq) to investigate phylogenetic relationships and species delimitation within the genus Leptastrea. We collected 77 colonies from nine localities of the Indo-Pacific and subjected them to genomic analyses. Based on de novo clustering, we obtained 44,162 SNPs (3701 loci) from the holobiont dataset and 62,728 SNPs (9573 loci) from the reads that map to coral transcriptome to reconstruct a robust phylogenetic hypothesis of the genus. Moreover, nearly complete mitochondrial genomes and ribosomal DNA arrays were retrieved by reference mapping. We combined concatenation-based phylogenetic analyses with coalescent-based species tree and species delimitation methods. Phylogenies suggest the presence of six distinct species, three corresponding to known taxa, namely Leptastrea bottae, Leptastrea inaequalis, Leptastrea transversa, one characterized by a remarkable skeletal variability encompassing the typical morphologies of Leptastrea purpurea and Leptastrea pruinosa, and two distinct and currently undescribed species. Therefore, based on the combination of genomic, morphological, morphometric, and distributional data, we herein described Leptastrea gibbosa sp. n. from the Pacific Ocean and Leptastrea magaloni sp. n. from the southwestern Indian Ocean and formally considered L. pruinosa as a junior synonym of L. purpurea. Notably, mitogenomes and rDNA yielded a concordant yet less resolved phylogeny reconstruction compared to the ones based on SNPs. This aspect demonstrates the strength and utility of RADseq technology for disentangling species boundaries in closely related species and in a challenging group such as scleractinian corals. 
PY 2020
PD AUG
SO Coral Reefs
SN 0722-4028
PU Springer Science and Business Media LLC
VL 39
IS 4
UT 000552022000014
BP 1001
EP 1025
DI 10.1007/s00338-020-01924-8
ID 73501
ER

EF