FN Archimer Export Format
PT J
TI High prevalence of Schistosoma haematobium x Schistosoma bovis hybrids in schoolchildren in Cote d'Ivoire
BT 
AF ANGORA, Etienne K.
   ALLIENNE, Jean-Francois
   REY, Olivier
   MENAN, Herve
   TOURE, Andre O.
   COULIBALY, Jean T.
   RASO, Giovanna
   YAVO, William
   N'GORAN, Eliezer K.
   UTZINGER, Jurg
   BALMER, Oliver
   BOISSIER, Jerome
AS 1:1,2,3;2:4;3:4;4:3;5:5;6:1,2,6,7;7:1,2;8:3;9:6,7;10:1,2;11:1,2;12:4;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;
C1 Swiss Trop & Publ Hlth Inst, Basel, Switzerland.
   Univ Basel, Basel, Switzerland.
   Univ Felix Houphouet Boigny, Unite Format & Rech Sci Pharmaceut & Biol, Abidjan, Cote Ivoire.
   Univ Perpignan Via Domitia, IFREMER, Univ Montpellier, IHPE,CNRS, Perpignan, France.
   Inst Pasteur Cote Ivoire, Abidjan, Cote Ivoire.
   Univ Felix Houphouet Boigny, Unite Format & Rech Biosci, Abidjan, Cote Ivoire.
   Ctr Suisse Rech Sci Cote Ivoire, Abidjan, Cote Ivoire.
C2 SWISS TPH, SWITZERLAND
   UNIV BASEL, SWITZERLAND
   UNIV FELIX HOUPHOUET BOIGNY, COTE IVOIRE
   UNIV PERPIGNAN, FRANCE
   INST PASTEUR COTE IVOIRE, COTE IVOIRE
   UNIV FELIX HOUPHOUET BOIGNY, COTE IVOIRE
   CTR SUISSE RECH SCI COTE IVOIRE, COTE IVOIRE
UM IHPE
IN WOS Cotutelle UMR
   copubli-int-hors-europe
   copubli-sud
IF 3.234
TC 25
UR https://archimer.ifremer.fr/doc/00624/73604/73060.pdf
LA English
DT Article
DE ;Cote d'Ivoire;hybrids;molecular analysis;Schistosoma bovis;Schistosoma haematobium
AB Schistosomiasis is a neglected tropical disease, though it is highly prevalent in many parts of sub-Saharan Africa. While Schistosoma haematobium-bovis hybrids have been reported in West Africa, no data about Schistosoma hybrids in humans are available from Cote d'Ivoire. This study aimed to identify and quantify S. haematobium-bovis hybrids among schoolchildren in four localities of Cote d'Ivoire. Urine samples were collected and examined by filtration to detect Schistosoma eggs. Eggs were hatched and 503 miracidia were individually collected and stored on Whatman(R) FTA cards for molecular analysis. Individual miracidia were molecularly characterized by analysis of mitochondrial cox1 and nuclear internal transcribed spacer 2 (ITS 2) DNA regions. A mitochondrial cox1-based diagnostic polymerase chain reaction was performed on 459 miracidia, with 239 (52.1%) exhibiting the typical band for S. haematobium and 220 (47.9%) the S. bovis band. The cox1 and ITS 2 amplicons were Sanger sequenced from 40 randomly selected miracidia to confirm species and hybrids status. Among the 33 cox1 sequences analysed, we identified 15 S. haematobium sequences (45.5%) belonging to seven haplotypes and 18 S. bovis sequences (54.5%) belonging to 12 haplotypes. Of 40 ITS 2 sequences analysed, 31 (77.5%) were assigned to pure S. haematobium, four (10.0%) to pure S. bovis and five (12.5%) to S. haematobium-bovis hybrids. Our findings suggest that S. haematobium-bovis hybrids are common in Cote d'Ivoire. Hence, intense prospection of domestic and wild animals is warranted to determine whether zoonotic transmission occurs. 
PY 2020
PD MAR
SO Parasitology
SN 0031-1820
PU Cambridge Univ Press
VL 147
IS 3
UT 000524935300004
BP 287
EP 294
DI 10.1017/S0031182019001549
ID 73604
ER

EF