FN Archimer Export Format
PT J
TI Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts
BT 
AF Estevez, Pablo
   Sibat, Manoella
   Leão-Martins, José Manuel
   Reis Costa, Pedro
   Gago-Martínez, Ana
   Hess, Philipp
AS 1:1;2:2;3:1;4:3;5:1;6:2;
FF 1:;2:PDG-ODE-DYNECO-PHYC;3:;4:;5:;6:PDG-ODE-DYNECO-PHYC;
C1 Biomedical Research Center (CINBIO), Department of Analytical and Food Chemistry, University of Vigo, Campus Universitario de Vigo, 36310 Vigo, Spain
   Ifremer, DYNECO, Laboratoire Phycotoxines, Rue de l’Île d’Yeu, 44311 Nantes, France
   IPMA—Portuguese Institute of the Sea and Atmosphere, Av. Brasília, 1449-006 Lisbon, Portugal
C2 CINBIO, SPAIN
   IFREMER, FRANCE
   IPMA, PORTUGAL
SI NANTES
SE PDG-ODE-DYNECO-PHYC
IN WOS Ifremer UPR
   DOAJ
   copubli-europe
IF 2.13
TC 23
UR https://archimer.ifremer.fr/doc/00624/73630/73067.pdf
   https://archimer.ifremer.fr/doc/00624/73630/73068.pdf
LA English
DT Article
DE ;ciguatoxins;HRMS;Q-TOF;ciguatera poisoning;C-CTX1;fragmentation pathways
AB Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed the initial confirmation of the presence of C-CTX1 in contaminated fish samples from the abovementioned areas, nevertheless the lack of commercially available reference materials for these particular ciguatoxin (CTX) analogues has been a major limitation to progress research. The EuroCigua project allowed the preparation of C-CTX1 laboratory reference material (LRM) from fish species (Seriola fasciata) from the Madeira archipelago (Portugal). This reference material was used to implement a liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) for the detection of C-CTX1, acquisition of full-scan as well as collision-induced mass spectra of this particular analogue. Fragmentation pathways were proposed based on fragments obtained. The optimized LC-HRMS method was then applied to confirm C-CTX1 in fish (Bodianus scrofa) caught in the Selvagens Islands (Portugal). 
PY 2020
PD APR
SO Toxins
SN 2072-6651
PU MDPI AG
VL 12
IS 4
UT 000533393900036
DI 10.3390/toxins12040267
ID 73630
ER

EF