FN Archimer Export Format
PT J
TI Encystment Induces Down-Regulation of an Acetyltransferase-Like Gene in Acanthamoeba castellanii
BT 
AF Rolland, Steven
   Mengue, Luce
   Noël, Cyril
   Crapart, Stéphanie
   Mercier, Anne
   Aucher, Willy
   Héchard, Yann
   Samba-Louaka, Ascel
AS 1:1;2:1;3:2;4:1;5:1;6:1;7:1;8:1;
FF 1:;2:;3:PDG-IRSI-SEBIMER;4:;5:;6:;7:;8:;
C1 UMR CNRS 7267 Ecologie et Biologie des Interactions, Université de Poitiers, Pôle Biologie-Santé, Bât. B36/B37, 1 rue Georges Bonnet, TSA 51106, 86073 Poitiers Cedex 9, France
   IFREMER-IRSI-Service de Bioinformatique (SeBiMER), Centre Bretagne, 1625 Route de Sainte-Anne, 29280 Plouzane, France
C2 UNIV POITIERS, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-IRSI-SEBIMER
IN WOS Ifremer UPR
   DOAJ
   copubli-france
   copubli-univ-france
IF 3.405
TC 4
UR https://archimer.ifremer.fr/doc/00625/73693/73214.pdf
   https://archimer.ifremer.fr/doc/00625/73693/73215.zip
LA English
DT Article
DE ;Acanthamoeba castellanii;encystment;N-acetyltransferase;lateral gene transfer;free-living amoebae
AB Acanthamoeba castellanii is a ubiquitous free-living amoeba. Pathogenic strains are causative agents of Acanthamoeba keratitis and granulomatous amoebic encephalitis. In response to adverse conditions, A. castellanii differentiate into cysts, which are metabolically inactive and resistant cells. This process, also named encystment, involves biochemical and genetic modifications that remain largely unknown. This study characterizes the role of the ACA1_384820 Acanthamoeba gene during encystment. This gene encodes a putative N-acetyltransferase, belonging to the Gcn5-related N-acetyltransferase (GNAT) family. We showed that expression of the ACA1_384820 gene was down-regulated as early as two hours after induction of encystment in A. castellanii. Interestingly, overexpression of the ACA1_384820 gene affects formation of cysts. Unexpectedly, the search of homologs of ACA1_384820 in the Eukaryota gene datasets failed, except for some species in the Acanthamoeba genus. Bioinformatics analysis suggested a possible lateral acquisition of this gene from prokaryotic cells. This study enabled us to describe a new Acanthamoeba gene that is down-regulated during encystment. 
PY 2020
PD MAY
SO Pathogens
SN 2076-0817
PU MDPI AG
VL 9
IS 5
UT 000541443700004
DI 10.3390/pathogens9050321
ID 73693
ER

EF