FN Archimer Export Format
PT J
TI Isolation and characterization of eight microsatellite loci from Galeocerdo cuvier (tiger shark) and cross-amplification in Carcharhinus leucas, Carcharhinus brevipinna, Carcharhinus plumbeus and Sphyrna lewini
BT 
AF PIROG, Agathe
   JAQUEMET, Sebastien
   BLAISON, Antonin
   SORIA, Marc
   MAGALON, Helene
AS 1:1;2:1;3:1,2;4:2;5:1;
FF 1:;2:;3:;4:;5:;
C1 Univ Reunion, UMR ENTROPIE, St Denis, Reunion, France.
   IRD Reunion, UMR MARBEC, St Denis, Reunion, France.
C2 UNIV LA REUNION, FRANCE
   IRD, FRANCE
UM MARBEC
IN DOAJ
IF 2.177
TC 11
UR https://archimer.ifremer.fr/doc/00626/73769/74405.pdf
   https://archimer.ifremer.fr/doc/00626/73769/74406.xlsx
LA English
DT Article
DE ;Carcharhiniform;Microsatellites;Control region;Population Genetics
AB The tiger shark Galeocerdo cuvier (Carcharhinidae) is a large elasmobranch suspected to have, as other apex predators, a keystone function in marine ecosystems and is currently considered Near Threatened (Red list IUCN). Knowledge on its ecology, which is crucial to design proper conservation and management plans, is very scarce. Here we describe the isolation of eight polymorphic microsatellite loci using 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries. Their characteristics were tested on a population of tiger shark (n = 101) from Reunion Island (South-Western Indian Ocean). All loci were polymorphic with a number of alleles ranging from two to eight. No null alleles were detected and no linkage disequilibrium was detected after Bonferroni correction. Observed and expected heterozygosities ranged from 0.03 to 0.76 and from 0.03 to 0.77, respectively. No locus deviated from Hardy-Weinberg equilibrium and the global F-IS of the population was of 0.04(NS). Some of the eight loci developed here successfully cross-amplified in the bull shark Carcharhinus leucas (one locus), the spinner shark Carcharhinus brevi pi n n a (four loci), the sandbar shark Carcharhinus plumbeus (five loci) and the scalloped hammerhead shark Sphyrna lewini (two loci). We also designed primers to amplify and sequence a mitochondrial marker, the control region. We sequenced 862 bp and found a low genetic diversity, with four polymorphic sites, a haplotype diversity of 0.15 and a nucleotide diversity of 2 x 10(-4). 
PY 2016
PD MAY
SO Peerj
SN 2167-8359
PU Peerj Inc
VL 4
UT 000376575200010
DI 10.7717/peerj.2041
ID 73769
ER

EF