FN Archimer Export Format PT J TI Toxic effects of leachates from plastic pearl-farming gear on embryo-larval development in the pearl oyster Pinctada margaritifera BT AF Gardon, Tony Huvet, Arnaud Paul-Pont, Ika Cassone, Anne-Laure Koua, Manaarii Sham Soyez, Claude Jezequel, Ronan Receveur, Justine Le Moullac, Gilles AS 1:1;2:2;3:4;4:4;5:1;6:1;7:3;8:3;9:1; FF 1:PDG-RBE-RMPF;2:PDG-RBE-PFOM-LPI;3:;4:;5:;6:PDG-RBE-RMPF;7:;8:;9:PDG-RBE-RMPF; C1 Ifremer, UMR EIO, UPF, ILM, IRD, F-98719, Taravao, Tahiti, French Polynesia Univ Brest, Ifremer, CNRS, IRD, LEMAR, F-29280, Plouzané, France CEDRE – 715 Rue Alain Colas, 29218, BREST Cedex 2, France Univ Brest, Ifremer, CNRS, IRD, LEMAR, F-29280, Plouzané, France C2 IFREMER, FRANCE IFREMER, FRANCE CEDRE, FRANCE CNRS, FRANCE SI TAHITI BREST SE PDG-RBE-RMPF PDG-RBE-PFOM-LPI UM LEMAR EIO IN WOS Ifremer UMR WOS Cotutelle UMR copubli-france IF 11.236 TC 47 UR https://archimer.ifremer.fr/doc/00627/73917/73257.pdf https://archimer.ifremer.fr/doc/00627/73917/73495.pdf LA English DT Article DE ;Aquaculture;Synthetic materials;Hazardous chemicals;Toxicity;Marine bivalve;Early development AB Pearl-farming leads to significant plastic pollution in French Polynesia (FP) as the end of life of most farming gear is currently poorly managed. Plastic debris released into the aquatic environment accumulates, with potentially detrimental effects on the lagoon ecosystem and pearl oyster Pinctada margaritifera, a species of ecological, commercial and social value. Here, we tested the effects of leachates from new (N) and aged (A) plastic pearl-farming gear (spat collector and synthetic rope) obtained after 24 h and 120 h incubation, on the embryo-larval development of the pearl oyster using an in-vitro assay. Embryos were exposed for 24 h and 48 h to a negative control (0) and the leachate from 0.1, 1, 10 and 100 g of plastic. L−1. After 24 h exposure to leachate at 100 g.L−1, effects were observed on embryo development (−38% to −60% of formed larvae) and mortality (+72% to +82%). Chemical analyses of plastic gear indicated the presence of 26 compounds, consisting of organic contaminants (PAHs) and additives (mainly phthalates). Screening of leachates demonstrated that these compounds leach into the surrounding seawater with an additional detection of pesticides. Higher levels of phthalates were measured in leachates obtained from new (6.7–9.1 μg.L−1) than from aged (0.4–0.5 μg.L−1) plastics, which could be part of the explanation of the clear difference in toxicity observed after 48 h exposure at lower concentrations (0.1–10 g.L−1), associated with mortality ranging from 26 to 86% and 17–28%, respectively. Overall, this study suggests that plastic gear used in the pearl-farming industry releases significant amounts of hazardous chemicals over their lifetime, which may affect pearl oyster development that call for in-situ exploration. PY 2020 PD JUN SO Water Research SN 0043-1354 PU Elsevier BV VL 179 UT 000537695500018 DI 10.1016/j.watres.2020.115890 ID 73917 ER EF