FN Archimer Export Format
PT J
TI Revival of ancient marine dinoflagellates using molecular biostimulation
BT 
AF Delebecq, Gaspard
   Schmidt, Sabine
   Ehrhold, Axel
   Latimier, Marie
   Siano, Raffaele
AS 1:1;2:2;3:3;4:4;5:4;
FF 1:;2:;3:PDG-REM-GM-LGS;4:PDG-ODE-DYNECO-PELAGOS;5:PDG-ODE-DYNECO-PELAGOS;
C1 Univ Brest, CNRS IRD, Ifremer, LEMAR F‐29280 Plouzané, France
   UMR5805 EPOC University of Bordeaux 33605 Pessac, France
   Ifremer, GM F‐29280 Plouzané ,France
   Ifremer, DYNECO F‐29280 Plouzané, France
C2 UBO, FRANCE
   UNIV BORDEAUX, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-REM-GM-LGS
   PDG-ODE-DYNECO-PELAGOS
UM LEMAR
IN WOS Ifremer UPR
   WOS Cotutelle UMR
   copubli-france
   copubli-univ-france
IF 2.923
TC 12
UR https://archimer.ifremer.fr/doc/00627/73960/73321.pdf
LA English
DT Article
DE ;Alexandrium;cyst;dinoflagellate;gibberellic acid;melatonin;paleoecology;priming;Scrippsiella;resurrection ecology
AB The biological processes involved in the preservation, viability, and revival of long‐term dormant dinoflagellate cysts buried in sediments remain unknown. Based on studies of plant seed physiology, we tested whether the revival of ancient cysts preserved in century‐old sediments from the Bay of Brest (France) could be stimulated by melatonin and gibberellic acid, two molecules commonly used in seed priming. Dinoflagellates were revived from sediments dated to approximately 150 years ago (156 ± 27, 32 cm depth), extending the known record age of cyst viability previously established as around one century. A culture suspension of sediments mixed with melatonin and gibberellic acid solutions as biostimulants exhibited germination of 11 dinoflagellate taxa that could not be revived under controlled culture conditions. The biostimulants revived some dinoflagellates from century‐old sediments, including the potentially toxic species Alexandrium minutum. The biostimulants showed positive effects on germination on even more ancient cysts, showing dose‐dependent effects on the germination of Scrippsiella acuminata. Concentrations of 1, 10, and 100 µM melatonin and gibberellic acid promoted germination. In contrast, 1000 µM solutions, particularly for melatonin, drastically decreased germination, suggesting a potential noxious effect of high doses of these molecules on dinoflagellate revival. Our findings suggest that melatonin and gibberellic acid are involved in the stimulation of germination of dinoflagellate cysts. These biostimulants can be used to germinate long‐term stored dinoflagellate cysts, which may promote studies of ancient strains in the resurrection ecology research field. 
PY 2020
PD AUG
SO Journal Of Phycology
SN 0022-3646
PU Wiley
VL 56
IS 4
UT 000536710800001
BP 1077
EP 1089
DI 10.1111/jpy.13010
ID 73960
ER

EF