External pH modulation during the growth of Vibrio tapetis, the aetiological agent of brown ring disease

Type Article
Date 2020-07
Language English
Author(s) Rahmani Alexandra1, Mathien Clémentine1, Bidault Adeline2, Le Goïc Nelly1, Paillard Christine1, Pichereau Vianney2
Affiliation(s) 1 : Univ Brest ,CNRS IRD, Ifremer UMR 6539 LEMAR F‐29280 Plouzané, France
2 : Univ Brest ,CNRS IRD, Ifremer UMR 6539 LEMAR F‐29280 Plouzané, France
Source Journal Of Applied Microbiology (1364-5072) (Wiley), 2020-07 , Vol. 129 , N. 1 , P. 3-16
DOI 10.1111/jam.14699
WOS© Times Cited 3
Note Special Issue: Fish and Shellfish Pathogens
Keyword(s) BRD, modulation effect, neutralizing activity, pH, pH modifications, Ruditapes philippinarum, Vibrio tapetis
Abstract

Aims

Brown Ring Disease (BRD) is an infection of the Manila clam Ruditapes philippinarum due to the pathogen Vibrio tapetis. During BRD, clams are facing immunodepression and shell biomineralization alteration. In this paper, we studied the role of pH on the growth of the pathogen and formulated hypothesis on the establishment of BRD by V. tapetis.

Methods and Results

In this study, we monitored the evolution of pH during the growth of V. tapetis in a range of pH and temperatures. We also measured the pH of Manila clam hemolymph and extrapallial fluids during infection by V. tapetis. We highlighted that V. tapetis modulates the external pH during its growth, to a value of 7.70. During the development of BRD, V. tapetis also influences extrapallial fluids and hemolymph pH in vitro in the first hours of exposure and in vivo after 3 days of infection.

Conclusions

Our experiments have shown a close interaction between V. tapetis CECT4600, a pathogen of Manila clam that induces BRD, and the pH of different compartments of the animals during infection. These results indicate that that the bacterium, through a direct mechanism or as a consequence of physiological changes encountered in the animal during infection, is able to interfere with the pH of Manila clam fluids. This pH modification might promote the infection process or at least create an imbalance within the animal, that would favor its persistence. This last hypothesis should be tested in future experiment.

Significance and Impact of Study

This study is the first observation of pH modifications in the context of BRD and might orient future research on the fine mechanisms of pH modulation associated to BRD.

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How to cite 

Rahmani Alexandra, Mathien Clémentine, Bidault Adeline, Le Goïc Nelly, Paillard Christine, Pichereau Vianney (2020). External pH modulation during the growth of Vibrio tapetis, the aetiological agent of brown ring disease. Journal Of Applied Microbiology, 129(1), 3-16. Publisher's official version : https://doi.org/10.1111/jam.14699 , Open Access version : https://archimer.ifremer.fr/doc/00629/74142/