FN Archimer Export Format
PT J
TI External pH modulation during the growth of Vibrio tapetis, the aetiological agent of brown ring disease
BT 
AF Rahmani, Alexandra
   Mathien, Clémentine
   Bidault, Adeline
   Le Goïc, Nelly
   Paillard, Christine
   Pichereau, Vianney
AS 1:1;2:1;3:2;4:1;5:1;6:2;
FF 1:;2:;3:;4:;5:;6:;
C1 Univ Brest ,CNRS IRD, Ifremer UMR 6539 LEMAR F‐29280 Plouzané, France
   Univ Brest ,CNRS IRD, Ifremer UMR 6539 LEMAR F‐29280 Plouzané, France
C2 CNRS, FRANCE
   UBO, FRANCE
UM LEMAR
IN WOS Cotutelle UMR
IF 3.772
TC 4
UR https://archimer.ifremer.fr/doc/00629/74142/73669.pdf
LA English
DT Article
DE ;BRD;modulation effect;neutralizing activity;pH;pH modifications;Ruditapes philippinarum;Vibrio tapetis
AB Aims Brown Ring Disease (BRD) is an infection of the Manila clam Ruditapes philippinarum due to the pathogen Vibrio tapetis. During BRD, clams are facing immunodepression and shell biomineralization alteration. In this paper, we studied the role of pH on the growth of the pathogen and formulated hypothesis on the establishment of BRD by V. tapetis. Methods and Results In this study, we monitored the evolution of pH during the growth of V. tapetis in a range of pH and temperatures. We also measured the pH of Manila clam hemolymph and extrapallial fluids during infection by V. tapetis. We highlighted that V. tapetis modulates the external pH during its growth, to a value of 7.70. During the development of BRD, V. tapetis also influences extrapallial fluids and hemolymph pH in vitro in the first hours of exposure and in vivo after 3 days of infection. Conclusions Our experiments have shown a close interaction between V. tapetis CECT4600, a pathogen of Manila clam that induces BRD, and the pH of different compartments of the animals during infection. These results indicate that that the bacterium, through a direct mechanism or as a consequence of physiological changes encountered in the animal during infection, is able to interfere with the pH of Manila clam fluids. This pH modification might promote the infection process or at least create an imbalance within the animal, that would favor its persistence. This last hypothesis should be tested in future experiment. Significance and Impact of Study This study is the first observation of pH modifications in the context of BRD and might orient future research on the fine mechanisms of pH modulation associated to BRD. 
PY 2020
PD JUN
SO Journal Of Applied Microbiology
SN 1364-5072
PU Wiley
VL 129
IS 1
UT 000541740900002
BP 3
EP 16
DI 10.1111/jam.14699
ID 74142
ER

EF