FN Archimer Export Format
PT J
TI Cultures of Dinophysis sacculus, D. acuminata and pectenotoxin 2 affect gametes and fertilization success of the Pacific oyster, Crassostrea gigas
BT 
AF Gaillard, Sylvain
   Le Goïc, Nelly
   MALO, Florent
   Boulais, Myrina
   Fabioux, Caroline
   Zaccagnini, Lucas
   CARPENTIER, Liliane
   Sibat, Manoella
   Réveillon, Damien
   Séchet, Veronique
   Hess, Philipp
   Hégaret, Helene
AS 1:1;2:4;3:1,2;4:;5:3;6:4;7:1;8:1;9:1;10:1;11:1;12:4;
FF 1:PDG-ODE-DYNECO-PHYC;2:;3:PDG-ODE-DYNECO-PHYC;4:PDG-RBE-PFOM-PI;5:;6:;7:PDG-ODE-DYNECO-PHYC;8:PDG-ODE-DYNECO-PHYC;9:PDG-ODE-DYNECO-PHYC;10:PDG-ODE-DYNECO-PHYC;11:PDG-ODE-DYNECO-PHYC;12:;
C1 IFREMER, DYNECO, Laboratoire Phycotoxines, F-44000, Nantes, France
   Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280, Plouzané, France
   Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280, Plouzané, France
   Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280, Plouzané, France
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   UBO, FRANCE
   CNRS, FRANCE
SI NANTES
   BREST
SE PDG-ODE-DYNECO-PHYC
   PDG-RBE-PFOM-PI
UM LEMAR
IN WOS Ifremer UPR
   WOS Ifremer UMR
   WOS Cotutelle UMR
   copubli-france
   copubli-univ-france
IF 8.071
TC 17
UR https://archimer.ifremer.fr/doc/00630/74167/73767.pdf
LA English
DT Article
DE ;Dinophysis spp.;Okadaic acid;Pectenotoxins;Oyster gametes;Fertilization success
AB Harmful algal blooms (HABs) of toxic species of the dinoflagellate genus Dinophysis are a threat to human health as they are mainly responsible for diarrheic shellfish poisoning (DSP) in the consumers of contaminated shellfish. Such contamination leads to shellfish farm closures causing major economic and social issues. The direct effects of numerous HAB species have been demonstrated on adult bivalves, whereas the effects on critical early life stages remain relatively unexplored. The present study aimed to determine the in vitro effects of either cultivated strains of D. sacculus and D. acuminata isolated from France or their associated toxins (i.e. okadaic acid (OA) and pectenotoxin 2 (PTX2)) on the quality of the gametes of the Pacific oyster Crassostrea gigas. This was performed by assessing the ROS production and viability of the gametes using flow cytometry, and fertilization success using microscopic counts. Oocytes were more affected than spermatozoa and their mortality and ROS production increased in the presence of D. sacculus and PTX2, respectively. A decrease in fertilization success was observed at concentrations as low as 0.5 cell mL−1 of Dinophysis spp. and 5 nM of PTX2, whereas no effect of OA could be observed. The effect on fertilization success was higher when both gamete types were concomitantly exposed compared to separate exposures, suggesting a synergistic effect. Our results also suggest that the effects could be due to cell-to-cell contact. These results highlight a potential effect of Dinophysis spp. and PTX2 on reproduction and recruitment of the Pacific oyster. 
PY 2020
PD OCT
SO Environmental Pollution
SN 0269-7491
PU Elsevier BV
VL 265
IS Part B
UT 000564557000012
DI 10.1016/j.envpol.2020.114840
ID 74167
ER

EF