FN Archimer Export Format
PT J
TI Genetic diversity and population structure of Tenacibaculum maritimum, a serious bacterial pathogen of marine fish: from genome comparisons to high throughput MALDI-TOF typing
BT 
AF Bridel, Sébastien
   Bourgeon, Frédéric
   Marie, Arnaud
   SAULNIER, Denis
   Pasek, Sophie
   Nicolas, Pierre
   Bernardet, Jean-François
   Duchaud, Eric
AS 1:1,2,3;2:4;3:2;4:5;5:6;6:7;7:1;8:1;
FF 1:;2:;3:;4:PDG-RBE-RMPF;5:;6:;7:;8:;
C1 Université Paris-Saclay, INRAE, UVSQ, VIM, 78350, Jouy-En-Josas, France
   Labofarm, Finalab, 22603, Loudéac, France
   Université de Versailles Saint-Quentin-En-Yvelines, 78180, Montigny-Le-Bretonneux, France
   Bio Chêne Vert, Finalab, Rue Blaise Pascal, 35220, Châteaubourg, France
   Ifremer, UMR EIO 241, Labex Corail, Centre du Pacifique, BP 49, Taravao, 98719, Tahiti, French Polynesia
   Institut de Systématique Evolution, Biodiversité, UMR 7205 Sorbonne Université MNHN CNRS EPHE, Paris, France
   Université Paris-Saclay, INRAE, MaIAGE, 78350, Jouy-en-Josas, France
C2 INRAE, FRANCE
   LABOFARM, FRANCE
   UNIV VERSAILLES, FRANCE
   BIO CHENE VERT, FRANCE
   IFREMER, FRANCE
   UNIV SORBONNE, FRANCE
   UNIV PARIS-SACLAY, FRANCE
SI TAHITI
SE PDG-RBE-RMPF
UM EIO
IN WOS Ifremer UMR
   DOAJ
   copubli-france
   copubli-p187
   copubli-univ-france
IF 3.683
TC 19
UR https://archimer.ifremer.fr/doc/00630/74174/73747.pdf
   https://archimer.ifremer.fr/doc/00630/74174/73748.pdf
   https://archimer.ifremer.fr/doc/00630/74174/73749.pdf
   https://archimer.ifremer.fr/doc/00630/74174/73750.pdf
   https://archimer.ifremer.fr/doc/00630/74174/73751.pdf
   https://archimer.ifremer.fr/doc/00630/74174/73752.pdf
   https://archimer.ifremer.fr/doc/00630/74174/73753.pdf
   https://archimer.ifremer.fr/doc/00630/74174/73754.pdf
   https://archimer.ifremer.fr/doc/00630/74174/73755.pdf
   https://archimer.ifremer.fr/doc/00630/74174/73756.pdf
LA English
DT Article
AB Tenacibaculum maritimum is responsible for tenacibaculosis, a devastating marine fish disease. This filamentous bacterium displays a very broad host range and a worldwide geographical distribution. We analyzed and compared the genomes of 25 T. maritimum strains, including 22 newly draft-sequenced genomes from isolates selected based on available MLST data, geographical origin and host fish. The genome size (~3.356 Mb in average) of all strains is very similar. The core genome is composed of 2116 protein-coding genes accounting for ~75% of the genes in each genome. These conserved regions harbor a moderate level of nucleotide diversity (~0.0071 bp−1) whose analysis reveals an important contribution of recombination (r/m ≥ 7) in the evolutionary process of this cohesive species that appears subdivided into several subgroups. Association trends between these subgroups and specific geographical origin or ecological niche remains to be clarified. We also evaluated the potential of MALDI-TOF-MS to assess the variability between T. maritimum isolates. Using genome sequence data, several detected mass peaks were assigned to ribosomal proteins. Additionally, variations corresponding to single or multiple amino acid changes in several ribosomal proteins explaining the detected mass shifts were identified. By combining nine polymorphic biomarker ions, we identified combinations referred to as MALDI-Types (MTs). By investigating 131 bacterial isolates retrieved from a variety of isolation sources, we identified twenty MALDI-Types as well as four MALDI-Groups (MGs). We propose this MALDI-TOF-MS Multi Peak Shift Typing scheme as a cheap, fast and an accurate method for screening T. maritimum isolates for large-scale epidemiological surveys. 
PY 2020
PD MAY
SO Veterinary Research
SN 0928-4249
PU Springer Science and Business Media LLC
VL 51
IS 1
UT 000533925600001
DI 10.1186/s13567-020-00782-0
ID 74174
ER

EF