FN Archimer Export Format
PT J
TI Diversity and Toxicity of the Genus Coolia Meunier in Brazil, and Detection of 44-Methyl Gambierone in Coolia tropicalis
BT 
AF Tibiriçá, Carlos Eduardo Junqueira de Azevedo
   Sibat, Manoella
   Fernandes, Luciano Felício
   Bilien, Gwenael
   Chomérat, Nicolas
   Hess, Philipp
   Mafra, Luiz L.
AS 1:1;2:2;3:3;4:4;5:4;6:2;7:1;
FF 1:;2:PDG-ODE-DYNECO-PHYC;3:;4:PDG-ODE-LITTORAL-LERBO;5:PDG-ODE-LITTORAL-LERBO;6:PDG-ODE-DYNECO-PHYC;7:;
C1 Universidade Federal do Paraná, Centro de Estudos do Mar, Cx, Postal 61, Pontal do Paraná PR 83255-976, Brazil
   IFREMER, DYNECO, Phycotoxins Laboratory, 44000 Nantes, France
   Universidade Federal do Paraná, Departamento de Botânica, Cx. Postal 19031, Curitiba PR 81531-990, Brazil
   Ifremer, LITTORAL, Laboratory of Environment and Resources Western Britanny, 29900 Concarneau France
C2 UNIV FED PARANA, BRAZIL
   IFREMER, FRANCE
   UNIV FED PARANA, BRAZIL
   IFREMER, FRANCE
SI NANTES
   CONCARNEAU
SE PDG-ODE-DYNECO-PHYC
   PDG-ODE-LITTORAL-LERBO
IN WOS Ifremer UPR
   DOAJ
   copubli-int-hors-europe
   copubli-sud
IF 2.13
TC 24
UR https://archimer.ifremer.fr/doc/00630/74186/73789.pdf
   https://archimer.ifremer.fr/doc/00630/74186/73790.pdf
LA English
DT Article
DE ;benthic microalgae;toxic dinoflagellates;toxicity assay;cooliatoxin;44-methyl gambierone
AB Coolia is a genus of marine benthic dinoflagellates which is widely distributed in tropical and temperate zones. Toxicity has been reported in selected Coolia species, although the identity of causative compounds is still controversial. In this study, we investigated the taxonomical and toxicological aspects of Coolia species from Brazil. Since light- and electron microscopy-based morphology was not enough to distinguish small-celled species, ITS and LSU D1-D3 phylogenetic analyses were used for species definition. Cultures of Coolia palmyrensis and Coolia santacroce were established from samples collected along the northeastern Brazilian coast, the first record of both species in South Atlantic waters. Cultures of Coolia malayensis and Coolia tropicalis were also established and exhibited acute in vivo toxicity to adults of Artemia salina, while C. palmyrensis and C. santacroce were non-toxic. The presence of 30 yessotoxin analogues, 7 metabolites of Coolia and 44 Gambierdiscus metabolites was screened in 14 strains of Coolia. 44-methyl gambierone (formerly referred to as MTX3) and a new isomer of this compound were detected only in C. tropicalis, using both low- and high-resolution LC-MS/MS. To our knowledge, this is the first report of gambierone analogues in dinoflagellates other than Gambierdiscus; the role of C. tropicalis in ciguatera poisoning thus deserves to be considered in further investigations. 
PY 2020
PD MAY
SO Toxins
SN 2072-6651
PU MDPI AG
VL 12
IS 5
UT 000541799400039
DI 10.3390/toxins12050327
ID 74186
ER

EF