Study of Synthesis Pathways of the Essential Polyunsaturated Fatty Acid 20:5n-3 in the Diatom Chaetoceros Muelleri Using 13C-Isotope Labeling

Type Article
Date 2020-05
Language English
Author(s) Remize Marine1, Planchon Frederic1, Loh Ai Ning2, Le Grand Fabienne5, Bideau Antoine1, Le Goic Nelly5, Fleury ElodieORCID3, Miner Philippe3, Corvaisier Rudolph5, Volety Aswani4, Soudant Philippe5
Affiliation(s) 1 : Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280 Plouzané, France
2 : Department of Earth and Ocean Sciences, Center for Marine Science, University of North Carolina Wilmington, 5600 Marvin K. Moss Ln, Wilmington, NC 28403, USA
3 : Institut Français de Recherche pour l’Exploitation de la Mer (IFREMER), Laboratoire des Sciences de l’Environnement Marin (LEMAR), UMR 6539 UBO/CNRS/IRD/IFREMER, CS 10070, 1625 Route de Sainte-Anne, 29280 Plouzane, France
4 : Department of Biology, Elon University, 50 Campus Drive, Elon, NC 27244, USA
5 : Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280 Plouzané, France
Source Biomolecules (2218-273X) (MDPI AG), 2020-05 , Vol. 10 , N. 5 , P. 797 (27p.)
DOI 10.3390/biom10050797
WOS© Times Cited 2
Note This article belongs to the Special Issue Lipids of Marine Algae
Keyword(s) synthesis pathway, diatom, 20:5n-3 (EPA), Chaetoceros muelleri, acyl-editing mechanism, compound-specific isotope analysis
Abstract

The present study sought to characterize the synthesis pathways producing the essential polyunsaturated fatty acid (PUFA) 20:5n-3 (EPA). For this, the incorporation of 13C was experimentally monitored into 10 fatty acids (FA) during the growth of the diatom Chaetoceros muelleri for 24 h. Chaetoceros muelleri preferentially and quickly incorporated 13C into C18 PUFAs such as 18:2n-6 and 18:3n-6 as well as 16:0 and 16:1n-7, which were thus highly 13C-enriched. During the experiment, 20:5n-3 and 16:3n-4 were among the least-enriched fatty acids. The calculation of the enrichment percentage ratio of a fatty acid B over its suspected precursor A allowed us to suggest that the diatom produced 20:5n-3 (EPA) by a combination between the n-3 (via 18:4n-3) and n-6 (via 18:3n-6 and 20:4n-6) synthesis pathways as well as the alternative ω-3 desaturase pathway (via 20:4n-6). In addition, as FA from polar lipids were generally more enriched in 13C than FA from neutral lipids, particularly for 18:1n-9, 18:2n-6 and 18:3n-6, the existence of acyl-editing mechanisms and connectivity between polar and neutral lipid fatty acid pools were also hypothesized. Because 16:3n-4 and 20:5n-3 presented the same concentration and enrichment dynamics, a structural and metabolic link was proposed between these two PUFAs in C. muelleri

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Remize Marine, Planchon Frederic, Loh Ai Ning, Le Grand Fabienne, Bideau Antoine, Le Goic Nelly, Fleury Elodie, Miner Philippe, Corvaisier Rudolph, Volety Aswani, Soudant Philippe (2020). Study of Synthesis Pathways of the Essential Polyunsaturated Fatty Acid 20:5n-3 in the Diatom Chaetoceros Muelleri Using 13C-Isotope Labeling. Biomolecules, 10(5), 797 (27p.). Publisher's official version : https://doi.org/10.3390/biom10050797 , Open Access version : https://archimer.ifremer.fr/doc/00630/74257/