FN Archimer Export Format
PT J
TI Study of Synthesis Pathways of the Essential Polyunsaturated Fatty Acid 20:5n-3 in the Diatom Chaetoceros Muelleri Using 13C-Isotope Labeling
BT 
AF Remize, Marine
   Planchon, Frederic
   Loh, Ai Ning
   Le Grand, Fabienne
   Bideau, Antoine
   Le Goic, Nelly
   Fleury, Elodie
   Miner, Philippe
   Corvaisier, Rudolph
   Volety, Aswani
   Soudant, Philippe
AS 1:1;2:1;3:2;4:5;5:1;6:5;7:3;8:3;9:5;10:4;11:5;
FF 1:;2:;3:;4:;5:;6:;7:PDG-RBE-PFOM-LPI;8:PDG-RBE-PFOM-LPI;9:;10:;11:;
C1 Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280 Plouzané, France
   Department of Earth and Ocean Sciences, Center for Marine Science, University of North Carolina Wilmington, 5600 Marvin K. Moss Ln, Wilmington, NC 28403, USA
   Institut Français de Recherche pour l’Exploitation de la Mer (IFREMER), Laboratoire des Sciences de l’Environnement Marin (LEMAR), UMR 6539 UBO/CNRS/IRD/IFREMER, CS 10070, 1625 Route de Sainte-Anne, 29280 Plouzane, France
   Department of Biology, Elon University, 50 Campus Drive, Elon, NC 27244, USA
   Univ Brest, CNRS, IRD, Ifremer, UMR 6539 LEMAR, F-29280 Plouzané, France
C2 UBO, FRANCE
   UNIV N CAROLINA, USA
   IFREMER, FRANCE
   UNIV ELON, USA
   CNRS, FRANCE
SI BREST
SE PDG-RBE-PFOM-LPI
UM LEMAR
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 4.694
TC 14
UR https://archimer.ifremer.fr/doc/00630/74257/73887.pdf
   https://archimer.ifremer.fr/doc/00630/74257/73888.zip
LA English
DT Article
DE ;synthesis pathway;diatom;20:5n-3 (EPA);Chaetoceros muelleri;acyl-editing mechanism;compound-specific isotope analysis
AB The present study sought to characterize the synthesis pathways producing the essential polyunsaturated fatty acid (PUFA) 20:5n-3 (EPA). For this, the incorporation of 13C was experimentally monitored into 10 fatty acids (FA) during the growth of the diatom Chaetoceros muelleri for 24 h. Chaetoceros muelleri preferentially and quickly incorporated 13C into C18 PUFAs such as 18:2n-6 and 18:3n-6 as well as 16:0 and 16:1n-7, which were thus highly 13C-enriched. During the experiment, 20:5n-3 and 16:3n-4 were among the least-enriched fatty acids. The calculation of the enrichment percentage ratio of a fatty acid B over its suspected precursor A allowed us to suggest that the diatom produced 20:5n-3 (EPA) by a combination between the n-3 (via 18:4n-3) and n-6 (via 18:3n-6 and 20:4n-6) synthesis pathways as well as the alternative ω-3 desaturase pathway (via 20:4n-6). In addition, as FA from polar lipids were generally more enriched in 13C than FA from neutral lipids, particularly for 18:1n-9, 18:2n-6 and 18:3n-6, the existence of acyl-editing mechanisms and connectivity between polar and neutral lipid fatty acid pools were also hypothesized. Because 16:3n-4 and 20:5n-3 presented the same concentration and enrichment dynamics, a structural and metabolic link was proposed between these two PUFAs in C. muelleri 
PY 2020
PD MAY
SO Biomolecules
SN 2218-273X
PU MDPI AG
VL 10
IS 5
UT 000545013700134
DI 10.3390/biom10050797
ID 74257
ER

EF