FN Archimer Export Format
PT J
TI Characterization of maitotoxin‐4 (MTX4) using electrospray positive mode ionization high‐resolution mass spectrometry and UV spectroscopy
BT 
AF Pisapia, Francesco
   Sibat, Manoella
   Watanabe, Ryuichi
   Roullier, Catherine
   Suzuki, Toshiyuki
   Hess, Philipp
   Herrenknecht, Christine
AS 1:1;2:1;3:2;4:3;5:2;6:1;7:3;
FF 1:PDG-ODE-DYNECO-PHYC;2:PDG-ODE-DYNECO-PHYC;3:;4:;5:;6:PDG-ODE-DYNECO-PHYC;7:;
C1 Ifremer, DYNECO Rue de l'Île d'Yeu 44311 Nantes, France
   NRIFS, Japan Fisheries Research and Education Agency 2‐12‐4 Fukuura, Kanazawa‐ku Yokohama Kanagawa 236‐8648, Japan
   Université de Nantes, MMS EA2160, Faculté de Pharmacie 9 rue Bias, 44035 Nantes, France
C2 IFREMER, FRANCE
   NRIFS, JAPAN
   UNIV NANTES, FRANCE
SI NANTES
SE PDG-ODE-DYNECO-PHYC
IN WOS Ifremer UPR
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 2.419
TC 7
UR https://archimer.ifremer.fr/doc/00633/74524/74355.pdf
LA English
DT Article
AB Rationale The dinoflagellate genera Gambierdiscus and Fukuyoa are producers of toxins responsible for Ciguatera Poisoning (CP). Although having very low oral potency, maitotoxins (MTXs) are very toxic following intraperitoneal injection and feeding studies have shown they may accumulate in fish muscle. To date, six MTX congeners have been described but two congeners (MTX2 and MTX4) have not yet been structurally elucidated. The aim of the present study was to further characterize MTX4. Methods Chemical analysis was performed using Liquid Chromatography coupled to a Diode Array Detector (DAD) and positive ionization mode High Resolution Mass Spectrometry (LC/HRMS) on partially purified extracts of G. excentricus (strain VGO792). HRMS/MS studies were also carried out to tentatively explain the fragmentation pathways of MTX and MTX4. Results The comparison of UV and HRMS (ESI+) spectra between MTX and MTX4 led us to propose the elemental formula of MTX4 (C157H241NO68S2, as unsalted molecule). The comparison of the theoretical and measured m/z values of the doubly charged ions of the isotopic profile in ESI+ were coherent with the proposed elemental formula of MTX4. The study of HRMS/MS spectra on the tri‐ammoniated adduct ([M–H+3NH4]2+) of both molecules gave additional information about structural features. The cleavage observed, probably located at C99–C100 in both MTX and MTX4, highlighted the same A‐side product ion shared by the two molecules. Conclusion All these investigations on the characterization of MTX4 contribute to highlighting that MTX4 belongs to the same structural family of MTXs. However to accomplish a complete structural elucidation of MTX4, NMR‐based study and LC/HRMSn investigation will have to be carried out. 
PY 2020
PD OCT
SO Rapid Communications In Mass Spectrometry
SN 0951-4198
PU Wiley
VL 34
IS 19
UT 000571215700004
DI 10.1002/rcm.8859
ID 74524
ER

EF