FN Archimer Export Format
PT J
TI Culture-Facilitated Comparative Genomics of the Facultative Symbiont Hamiltonella defensa
BT 
AF CHEVIGNON, Germain
   BOYD, Bret M.
   BRANDT, Jayce W.
   OLIVER, Kerry M.
   STRAND, Michael R.
AS 1:1;2:1;3:1;4:1;5:1;
FF 1:;2:;3:;4:;5:;
C1 Univ Georgia, Dept Entomol, Athens, GA 30602 USA.
C2 UNIV GEORGIA, USA
IN DOAJ
IF 3.726
TC 32
UR https://archimer.ifremer.fr/doc/00635/74665/74590.pdf
   https://archimer.ifremer.fr/doc/00635/74665/74591.zip
LA English
DT Article
DE ;insect;aphid;bacteria;comparative genomics;parasitoid;defense
AB Many insects host facultative, bacterial symbionts that confer conditional fitness benefits to their hosts. Hamiltonella defensa is a common facultative symbiont of aphids that provides protection against parasitoid wasps. Protection levels vary among strains of H. defensa that are also differentially infected by bacteriophages named APSEs. However, little is known about trait variation among strains because only one isolate has been fully sequenced. Generating complete genomes for facultative symbionts is hindered by relatively large genome sizes but low abundances in hosts like aphids that are very small. Here, we took advantage of methods for culturing H. defensa outside of aphids to generate complete genomes and transcriptome data for four strains of H. defensa from the pea aphid Acyrthosiphon pisum. Chosen strains also spanned the breadth of the H. defensa phylogeny and differed in strength of protection conferred against parasitoids. Results indicated that strains shared most genes with roles in nutrient acquisition, metabolism, and essential housekeeping functions. In contrast, the inventory of mobile genetic elements varied substantially, which generated strain specific differences in gene content and genome architecture. In some cases, specific traits correlated with differences in protection against parasitoids, but in others high variation between strains obscured identification of traits with likely roles in defense. Transcriptome data generated continuous distributions to genome assemblies with some genes that were highly expressed and others that were not. Single molecule real-time sequencing further identified differences in DNA methylation patterns and restriction modification systems that provide defense against phage infection. 
PY 2018
PD MAR
SO Genome Biology And Evolution
SN 1759-6653
PU Oxford Univ Press
VL 10
IS 3
UT 000429483700009
BP 786
EP 802
DI 10.1093/gbe/evy036
ID 74665
ER

EF