TY - JOUR T1 - Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta A1 - Kanost,Michael R. A1 - Arrese,Estela L. A1 - Cao,Xiaolong A1 - Chen,Yun-Ru A1 - Chellapilla,Sanjay A1 - Goldsmith,Marian R. A1 - Grosse-Wilde,Ewald A1 - Heckel,David G. A1 - Herndon,Nicolae A1 - Jiang,Haobo A1 - Papanicolaou,Alexie A1 - Qu,Jiaxin A1 - Soulages,Jose L. A1 - Vogel,Heiko A1 - Walters,James A1 - Waterhouse,Robert M. A1 - Ahn,Seung-Joon A1 - Almeida,Francisca C. A1 - An,Chunju A1 - Aqrawi,Peshtewani A1 - Bretschneider,Anne A1 - Bryant,William B. A1 - Bucks,Sascha A1 - Chao,Hsu A1 - Chevignon,Germain A1 - Christen,Jayne M. A1 - Clarke,David F. A1 - Dittmer,Neal T. A1 - Ferguson,Laura C. F. A1 - Garavelou,Spyridoula A1 - Gordon,Karl H. J. A1 - Gunaratna,Ramesh T. A1 - Han,Yi A1 - Hauser,Frank A1 - He,Yan A1 - Heidel-Fischer,Hanna A1 - Hirsh,Ariana A1 - Hu,Yingxia A1 - Jiang,Hongbo A1 - Kalra,Divya A1 - Klinner,Christian A1 - Konig,Christopher A1 - Kovar,Christie A1 - Kroll,Ashley R. A1 - Kuwar,Suyog S. A1 - Lee,Sandy L. A1 - Lehman,Rudiger A1 - Li,Kai A1 - Li,Zhaofei A1 - Liang,Hanquan A1 - Lovelace,Shanna A1 - Lu,Zhiqiang A1 - Mansfield,Jennifer H. A1 - McCulloch,Kyle J. A1 - Mathew,Tittu A1 - Morton,Brian A1 - Muzny,Donna M. A1 - McCulloch,Kyle J. A1 - Mathew,Tittu A1 - Morton,Brian A1 - Muzny,Donna M. A1 - Neunemann,David A1 - Ongeri,Fiona A1 - Pauchet,Yannick A1 - Pu,Ling -Ling A1 - Pyrousis,Ioannis A1 - Rao,Xiang -Jun A1 - Redding,Amanda A1 - Roesel,Charles A1 - Sanchez-Gracia,Alejandro A1 - Schaack,Sarah A1 - Shukla,Aditi A1 - Tetreau,Guillaume A1 - Wang,Yang A1 - Xiong,Guang-Hua A1 - Traut,Walther A1 - Walsh,Tom K. A1 - Worley,Kim C. A1 - Wu,Di A1 - Wu,Wenbi A1 - Wu,Yuan-Qing A1 - Zhang,Xiufeng A1 - Zou,Zhen A1 - Zucker,Hannah A1 - Briscoe,Adriana D. A1 - Burmester,Thorsten A1 - Clem,Rollie J. A1 - Feyereisen,Rene A1 - Grimmelikhuijzen,Cornelis J. P. A1 - Hamodrakas,Stavros J. A1 - Hansson,Bill S. A1 - Huguet,Elisabeth A1 - Jermiin,Lars S. A1 - Lan,Que A1 - Lehman,Herman K. A1 - Lorenzen,Marce A1 - Merzendorfer,Hans A1 - Michalopoulos,Ioannis A1 - Morton,David B. A1 - Muthukrishnan,Subbaratnam A1 - Oakeshott,John G. A1 - Palmer,Will A1 - Park,Yoonseong A1 - Passarelli,A. Lorena A1 - Rozas,Julio A1 - Schwartz,Lawrence M. A1 - Smith,Wendy A1 - Southgate,Agnes A1 - Vilcinskas,Andreas A1 - Vogt,Richard A1 - Wang,Ping A1 - Werren,John A1 - Yu,Xiao-Qiang A1 - Zhou,Jing-Jiang A1 - Brown,Susan J. A1 - Scherer,Steven E. A1 - Richards,Stephen A1 - Blissard,Gary W. AD - Kansas State Univ, Dept Biochem & Mol Biophys, Manhattan, KS 66506 USA. AD - Oklahoma State Univ, Dept Biochem & Mol Biol, Stillwater, OK 74078 USA. AD - Oklahoma State Univ, Dept Entomol & Plant Pathol, Stillwater, OK 74078 USA. AD - Cornell Univ, Boyce Thompson Inst, Tower Rd, Ithaca, NY 14853 USA. AD - Kansas State Univ, Div Biol, KSU Bioinformat Ctr, Manhattan, KS 66506 USA. AD - Univ Rhode Isl, Dept Biol Sci, Kingston, RI 02881 USA. AD - Max Planck Inst Chem Ecol, Dept Evolutionary Neuroethol, Hans Knoell Str 8, D-07745 Jena, Germany. AD - Max Planck Inst Chem Ecol, Dept Entomol, Hans Knoell Strasse 8, D-07745 Jena, Germany. AD - Univ Western Sydney, Hawkesbury Inst Environm, Richmond, NSW 2753, Australia. AD - Baylor Coll Med, Human Genome Sequencing Ctr, 1 Baylor Plaza, Houston, TX 77030 USA. AD - Univ Kansas, Dept Ecol & Evolutionary Biol, Lawrence, KS 66045 USA. AD - Univ Geneva, Dept Genet Med & Dev, Sch Med, Rue Michel Servet 1, CH-1211 Geneva, Switzerland. AD - Swiss Inst Bioinformat, Rue Michel Servet 1, CH-1211 Geneva, Switzerland. AD - MIT, Comp Sci & Artificial Intelligence Lab, 32 Vassar St, Cambridge, MA 02139 USA. AD - Broad Inst MIT & Harvard, 415 Main St, Cambridge, MA 02142 USA. AD - Univ Barcelona, Dept Genet, Barcelona, Spain. AD - Univ Barcelona, Inst Recerca Biodiversitat IRBio, Barcelona, Spain. AD - China Agr Univ, Dept Entomol, Beijing, Peoples R China. AD - Kansas State Univ, Div Biol, Manhattan, KS 66506 USA. AD - Univ Tours, UMR CNRS 7261, Inst Rech Biol IInsecte, UFR Sci & Techn, Tours, France. AD - CSIRO Land & Water, Clunies Ross St, Canberra, ACT 2601, Australia. AD - Dept Zool, South Parks Rd, Oxford, England. AD - Acad Athens, Biomed Res Fdn, Ctr Syst Biol, Athens, Greece. AD - CSIRO Hlth & Biosecur, Clunies Ross St,Acton,2601, Acton, ACT, Australia. AD - Univ Copenhagen, Ctr Funct & Comparat Insect Genom, Dept Biol, Univ Pk 15, DK-2100 Copenhagen, Denmark. AD - Columbia Univ, Dept Biol, Barnard Coll, 3009 Broadway, New York, NY 10027 USA. AD - Southwest Univ, Coll Plant Protect, Key Lab Entomol & Pest Control Engn, Chongqing 400715, Peoples R China. AD - Dept Biol, Reed Coll, Portland, OR 97202 USA. AD - Fraunhofer Inst Mol Biol & Appl Ecol IME, Bioresources Project Grp, Winchesterstr 2, D-35394 Giessen, Germany. AD - Donghua Univ, Coll Chem Chem Engn & Biotechnol, Shanghai 201620, Peoples R China. AD - Northwest A&F Univ, Coll Plant Protect, Yangling 712100, Shaanxi, Peoples R China. AD - UT Southwestern Med Ctr, McDermott Ctr Human Growth & Dev, 5323 Harry Hines Blvd, Dallas, TX 75390 USA. AD - Univ So Maine, Dept Biol Sci, Portland, ME 04104 USA. AD - Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92697 USA. AD - Anhui Agr Univ, Sch Plant Protect, Hefei, Anhui, Peoples R China. AD - Univ Rochester, Dept Biol, Rochester, NY 14627 USA. AD - Northeastern Univ, Dept Marine & Environm Sci, Boston, MA 02115 USA. AD - Cornell Univ, Dept Entomol, New York State Agr Expt Stn, Geneva, NY 14456 USA. AD - Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing, Peoples R China. AD - Med Univ Lubeck, Inst Biol, D-23538 Lubeck, Germany. AD - Hamilton Coll, Neurosci Program, Clinton, NY 13323 USA. AD - Univ Hamburg, Inst Zool, Hamburg, Germany. AD - Univ Ghent, Fac Bioscience Engn, Dept Crop Protect, Ghent, Belgium. AD - Univ Athens, Dept Cell Biol & Biophys, Fac Biol, Athens, Greece. AD - Univ Wisconsin, Dept Entomol, Madison, WI USA. AD - Hamilton Coll, Biol Dept & Neurosci Program, Clinton, NY 13323 USA. AD - North Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. AD - Univ Siegen, Inst Biol Mol Biol, Sch Nat Sci & Engn, Adolf Reichwein Str 2,AR C3010, D-57076 Siegen, Germany. AD - Oregon Hlth & Sci Univ, Sch Dent, Dept Integrat Biosciences, 3181 SW Sam Jackson Pk Rd,BRB421,L595, Portland, OR 97239 USA. AD - Univ Cambridge, Dept Genet, Downing St, Cambridge CB2 3EH, England. AD - Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. AD - Univ Massachusetts, Dept Biol, Amherst, MA 01003 USA. AD - Northeastern Univ, Dept Biol, Boston, MA 02115 USA. AD - Coll Charleston, Dept Biol, Charleston, SC 29424 USA. AD - Justus Liebig Univ, Inst Insect Biotechnol, Heinrich Buff Ring 26-32, D-35392 Giessen, Germany. AD - Univ South Carolina, Dept Biol Sci, Columbia, SC 29205 USA. AD - Univ Missouri Kansas City, 5007 Rockhill Rd, Kansas City, MO 64110 USA. AD - Rothamsted Res, Dept Biol Chem & Crop Protect, Harpenden AL5 2JQ, Herts, England. UR - https://doi.org/10.1016/j.ibmb.2016.07.005 DO - 10.1016/j.ibmb.2016.07.005 KW - Lepidoptera KW - Insect KW - Tobacco hornworm KW - Synteny KW - Moth KW - Insect biochemistry KW - Innate immunity N2 - Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages. The Msex_1.0 genome assembly resulted in a total genome size of 419.4 Mbp. Repetitive sequences accounted for 25.8% of the assembled genome. The official gene set is comprised of 15,451 protein-coding genes, of which 2498 were manually curated. Extensive RNA-seq data from many tissues and developmental stages were used to improve gene models and for insights into gene expression patterns. Genome wide synteny analysis indicated a high level of macrosynteny in the Lepidoptera. Annotation and analyses were carried out for gene families involved in a wide spectrum of biological processes, including apoptosis, vacuole sorting, growth and development, structures of exoskeleton, egg shells, and muscle, vision, chemosensation, ion channels, signal transduction, neuropeptide signaling, neurotransmitter synthesis and transport, nicotine tolerance, lipid metabolism, and immunity. This genome sequence, annotation, and analysis provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects. Y1 - 2016/09 PB - Pergamon-elsevier Science Ltd JF - Insect Biochemistry And Molecular Biology SN - 0965-1748 VL - 76 SP - 118 EP - 147 ID - 74667 ER -