FN Archimer Export Format
PT J
TI Methanohalophilus profundi sp. nov., a methylotrophic halophilic piezophilic methanogen isolated from a deep hypersaline anoxic basin
BT 
AF L'HARIDON, Stephane
   Haroun, Hani
   Corre, Erwan
   Roussel, Erwan
   Chalopin, Morgane
   Pignet, Patricia
   Balière, Charlotte
   la Cono, Violetta
   Jebbar, Mohamed
   Yakimov, Michail
   Toffin, Laurent
AS 1:4;2:4;3:2;4:1;5:1;6:1;7:1;8:3;9:4;10:3;11:1;
FF 1:;2:;3:;4:PDG-REM-EEP-LMEE;5:;6:PDG-REM-EEP-LMEE;7:;8:;9:;10:;11:PDG-REM-EEP-LMEE;
C1 Univ Brest (UBO), Ifremer, CNRS, Laboratoire de Microbiologie des Environnements Extrêmes, F-29280 Plouzané, France
   Sorbonne Université, CNRS, Station Biologique de Roscoff, Plateforme ABiMS, F-29688 Roscoff, France
   Institute for Coastal Marine Environment CNR, 98122 Messina, Italy
   Univ Brest (UBO), Ifremer, CNRS, Laboratoire de Microbiologie des Environnements Extrêmes, F-29280 Plouzané, France
C2 IFREMER, FRANCE
   UNIV SORBONNE, FRANCE
   CNR IAMC, ITALY
   UBO, FRANCE
SI BREST
SE PDG-REM-EEP-LMEE
UM BEEP-LM2E
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   copubli-france
   copubli-europe
   copubli-univ-france
IF 4.022
TC 5
UR https://archimer.ifremer.fr/doc/00635/74744/75013.pdf
LA English
DT Article
DE ;Methanohalophilus;Methanosarcinaceae;Methanosarcinales;Methylotrophic;Piezophilic;Deep hypersalin anoxic basin (DHAB)
AB A novel anaerobic methylotrophic halophilic methanogen strain SLHTYROT was isolated from a deep hypersaline anoxic basin called “Tyro” located in the Eastern Mediterranean Sea. Cells of SLHTYROT were motile cocci. The strain SLHTYROT grew between 12 and 37 °C (optimum 30 °C), at pH between 6.5 and 8.2 (optimum pH 7.5) and salinity from 45 to 240 g L−1 NaCl (optimum 135 g L−1). Strain SLHTYROT was methylotrophic methanogen able to use methylated compounds (trimethylamine, dimethylamine, monomethylamine and methanol). Strain SLHTYROT was able to grow at in situ hydrostatic pressure and temperature conditions (35 MPa, 14 °C). Phylogenetic analysis based on 16S rRNA gene and mcrA gene sequences indicated that strain SLHTYROT was affiliated to genus Methanohalophilus within the order Methanosarcinales. It shared >99.16% of the 16S rRNA gene sequence similarity with strains of other Methanohalophilus species. Based on ANIb, AAI and dDDH measurements, and the physiological properties of the novel isolate, we propose that strain SLHTYROT should be classified as a representative of a novel species, for which the name Methanohalophilus profundi sp. nov. is proposed; the type strain is SLHTYROT (=DSM 108854 = JCM 32768 = UBOCC-M-3308). 
PY 2020
PD SEP
SO Systematic And Applied Microbiology
SN 0723-2020
PU Elsevier BV
VL 43
IS 5
UT 000574879000004
DI 10.1016/j.syapm.2020.126107
ID 74744
ER

EF