FN Archimer Export Format
PT J
TI Unveiling protist diversity associated with the Pacific oyster Crassostrea gigas using blocking and excluding primers
BT 
AF Clerissi, Camille
   Guillou, Laure
   Escoubas, Jean Michel
   Toulza, Eve
AS 1:1,2;2:3;3:4;4:5;
FF 1:PDG-RBE-IHPE;2:;3:;4:;
C1 IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Perpignan, France
   PSL Université Paris: EPHE-UPVD-CNRS, USR 3278 CRIOBE, Université de Perpignan, 52 Avenue Paul Alduy, 66860, Perpignan Cedex, France
   Sorbonne Université, CNRS, UMR7144 Adaptation et Diversité en Milieu Marin, Ecology of Marine Plankton (ECOMAP), Station Biologique de Roscoff SBR, Roscoff, France
   IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Montpellier, France
   IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Perpignan, France
C2 IFREMER, FRANCE
   EPHE, FRANCE
   UNIV SORBONNE, FRANCE
   CNRS, FRANCE
   UNIV PERPIGNAN, FRANCE
SI MONTPELLIER
SE PDG-RBE-IHPE
UM IHPE
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-univ-france
IF 3.605
TC 5
UR https://archimer.ifremer.fr/doc/00636/74799/75165.pdf
   https://archimer.ifremer.fr/doc/00636/74799/75166.xls
   https://archimer.ifremer.fr/doc/00636/74799/75167.tiff
   https://archimer.ifremer.fr/doc/00636/74799/75168.xls
   https://archimer.ifremer.fr/doc/00636/74799/75169.tiff
   https://archimer.ifremer.fr/doc/00636/74799/75170.xls
   https://archimer.ifremer.fr/doc/00636/74799/75171.xls
   https://archimer.ifremer.fr/doc/00636/74799/75172.tiff
   https://archimer.ifremer.fr/doc/00636/74799/75173.xls
LA English
DT Article
DE ;Holobiont;Metabarcoding;Microbiome;Ostreoida;Crassostrea
AB Background Microbiome of macroorganisms might directly or indirectly influence host development and homeostasis. Many studies focused on the diversity and distribution of prokaryotes within these assemblages, but the eukaryotic microbial compartment remains underexplored so far. Results To tackle this issue, we compared blocking and excluding primers to analyze microeukaryotic communities associated with Crassostrea gigas oysters. High-throughput sequencing of 18S rRNA genes variable loops revealed that excluding primers performed better by not amplifying oyster DNA, whereas the blocking primer did not totally prevent host contaminations. However, blocking and excluding primers showed similar pattern of alpha and beta diversities when protist communities were sequenced using metabarcoding. Alveolata, Stramenopiles and Archaeplastida were the main protist phyla associated with oysters. In particular, Codonellopsis, Cyclotella, Gymnodinium, Polarella, Trichodina, and Woloszynskia were the dominant genera. The potential pathogen Alexandrium was also found in high abundances within some samples. Conclusions Our study revealed the main protist taxa within oysters as well as the occurrence of potential oyster pathogens. These new primer sets are promising tools to better understand oyster homeostasis and disease development, such as the Pacific Oyster Mortality Syndrome (POMS) targeting juveniles. 
PY 2020
PD JUN
SO Bmc Microbiology
SN 1471-2180
PU Springer Science and Business Media LLC
VL 20
IS 1
UT 000548771400002
DI 10.1186/s12866-020-01860-1
ID 74799
ER

EF