FN Archimer Export Format PT J TI An eDNA/eRNA‐based approach to investigate the life cycle of non‐cultivable shellfish micro‐parasites: the case of Bonamia ostreae , a parasite of the European flat oyster Ostrea edulis BT AF Mérou, Nicolas Lecadet, Cyrielle Pouvreau, Stephane Arzul, Isabelle AS 1:1;2:1;3:2;4:1; FF 1:PDG-RBE-SGMM-LGPMM;2:PDG-RBE-SGMM-LGPMM;3:PDG-RBE-PFOM-LPI;4:PDG-RBE-SGMM-LGPMM; C1 Laboratoire de Génétique et Pathologie des Mollusques Marins Ifremer SG2M‐LGPMM Avenue de Mus de Loup 17390 La Tremblade France Laboratoire des Sciences de l'Environnement Marin UMR 6539, Ifremer/UBO/IRD/CNRS Ifremer 11 Presqu'île du Vivier 29840 Argenton‐en‐Landunvez France C2 IFREMER, FRANCE IFREMER, FRANCE SI LA TREMBLADE ARGENTON SE PDG-RBE-SGMM-LGPMM PDG-RBE-PFOM-LPI UM LEMAR IN WOS Ifremer UPR WOS Ifremer UMR DOAJ IF 5.813 TC 18 UR https://archimer.ifremer.fr/doc/00636/74800/75174.pdf LA English DT Article AB Environmental DNA approaches are increasingly used to detect microorganisms in environmental compartments, including water. They show considerable advantages to study non‐cultivable microorganisms like Bonamia ostreae, a protozoan parasite inducing significant mortality in populations of flat oyster Ostrea edulis . Although B. ostreae development within the host has been well described, questions remain about its behaviour in the environment. As B. ostreae transmission is direct, seawater appears as an interesting target to develop early detection tools and improve our understanding of disease transmission mechanisms. In this context, we have developed an eDNA/eRNA approach allowing detecting and quantifying B. ostreae 18S rDNA/rRNA as well as monitoring its presence in seawater by real‐time PCR. B. ostreae DNA could be detected up to 4 days while RNA could be detected up to 30 days, suggesting a higher sensitivity of the eRNA‐based tool. Additionally, more than 90% of shed parasites were no longer detected after 2 days outside the oysters. By allowing B. ostreae detection in seawater, this approach would not only be useful to monitor the presence of the parasite in oyster production areas but also to evaluate the effect of changing environmental factors on parasite survival and transmission. PY 2020 PD NOV SO Microbial Biotechnology SN 1751-7915 PU Wiley VL 13 IS 6 UT 000544335600001 BP 1807 EP 1818 DI 10.1111/1751-7915.13617 ID 74800 ER EF