FN Archimer Export Format
PT Rapport
TI Stereology reading protocol when using quantitative histology for the determination of sexual maturity in fish ovaries
OT Protocole de lectures stéréologiques pour l’utilisation d’histologie quantitative dans la détermination des phases de maturité sexuelle chez les ovaires de poissons
BT 
AF DUBROCA, Laurent
   ELIE, Nicolas
   HEUDE-BERTHELIN, Clothilde
   KELLNER, Kristell
   LE MELEDER, Anna
   LEPOITTEVIN, Mélanie
   MARTIN, Valentine
   NIVET, Timothe
   SAUGER, Carine
   VILLAIN-NAUD, Nadège
AS 1:1;2:3;3:2;4:2;5:1;6:2;7:1;8:1;9:1;10:2;
FF 1:PDG-RBE-HMMN-LRHPB;2:;3:;4:;5:PDG-RBE-HMMN-LRHPB;6:;7:PDG-RBE-HMMN-LRHPB;8:PDG-RBE-HMMN-LRHPB;9:PDG-RBE-HMMN-LRHPB;10:;
C1 Institut Français de Recherche pour l’Exploitation de la Mer, IFREMER, Laboratoire Ressources Halieutiques de Port-en-Bessin, 14520 Port-en-Bessin-Huppain, France
   University of Caen Normandy, Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) MNHN, Sorbonne Université, UCN, CNRS-7208, IRD, UA, team EMERGE, esplanade de la Paix 14032 Caen, France
   Normandie Univ, UNICAEN, SF 4206 ICORE, CMABIO3, 14000 Caen, France
C2 IFREMER, FRANCE
   UNIV CAEN NORMANDIE, FRANCE
   UNIV NORMANDIE, FRANCE
SI PORT-EN-BESSIN
SE PDG-RBE-HMMN-LRHPB
UR https://archimer.ifremer.fr/doc/00640/75173/102226.pdf
LA English
DT Report
AB This protocol was established for the evaluation of sexual maturity for exploited stock species, using quantitative histology (stereology). This document describes the reading method used on histological slides under the cross-platform QuPath.           This document follows the work of different workshops that aimed to improve the compilation of data on sexual maturity. The WKMATCH (WorKshop for MATurity staging CHairs, 2012) and the WKASMSF (Workshop for Advancing Sexual Maturity Staging in Fish, 2018) defined a universal evaluation grid for sexual maturity staging of different species, including teleosteans. During the WKMATCH workshop, two main recommendations were made, underlining the need to improve and complete knowledge on sexual maturity, as well as to harmonize the practices used to determine these sexual phases.           Another workshop, the WKMATHIS (WorKshop on sexual MATurity staging from HIStological tools) that took place in Caen in 2017, added new objectives : review the current knowledge on gonad histology, determine a sexual maturity scale at a cellular level, and histological studies should be made in order to improve the determination of sexual maturity on a macroscopic scale.            This protocol, drawn from these workshops and their conclusions, offers guidelines on how to read histological slides through the use of stereology. As of June 2022, this protocol is linked to the MATO (MATurité Objectif des poissons par l’histologie quantitative) project, a study restricted to female gonad and the different cellular structures found inside the ovaries for different species (Pleuronectes platessa, Lepidorhombus whiffiagonis, Lepidorhombus boscii, Mullus surmuletus and Micromesistius poutassou).           This protocol will present reading methods that favor an objective reading of histological slides, through stereology, and will allow less experienced readers to partake in this exercise. For a detailed description of the germinal cells, refer to the lexicon of the studied specie 
PY 2023
PD MAY
DI 10.13155/75173
ID 75173
ER

EF