FN Archimer Export Format
PT J
TI Accumulation curves of environmental DNA sequences predict coastal fish diversity in the coral triangle
BT 
AF Juhel, Jean-Baptiste
   Utama, Rizkie S.
   Marques, Virginie
   Vimono, Indra B.
   Sugeha, Hagi Yulia
   Pouyaud, Laurent
   Dejean, Tony
   Mouillot, David
   Hocdé, Régis
AS 1:1;2:2;3:1;4:2;5:2;6:3;7:4;8:5;9:1,6;10:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;
C1 MARBEC, Univ. Montpellier, CNRS, Ifremer, IRD, Montpellier, France
   Research Center for Oceanography, Indonesian Institute of Sciences, Jl. Pasir Putih 1, Ancol Timur, Jakarta Utara, Indonesia
   Politeknik Kelautan dan Perikanan Sorong, KKD BP Sumberdaya Genetik, Konservasi dan Domestikasi, Papua Barat 98411, Indonesia
   Institut des Sciences de l'Evolution de Montpellier, Montpellier, France
   SPYGEN, 73370 Le Bourget-du-Lac, France
   ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Australia
C2 UNIV MONTPELLIER, FRANCE
   LIPI, INDONESIA
   POLITEKNIK KELAUTAN DAN PERIKANAN SORONG, INDONESIA
   ISEM, FRANCE
   SPYGEN, FRANCE
   UNIV JAMES COOK, AUSTRALIA
UM MARBEC
IN WOS Cotutelle UMR
   copubli-france
   copubli-int-hors-europe
   copubli-sud
IF 5.349
TC 26
UR https://archimer.ifremer.fr/doc/00640/75232/79601.pdf
LA English
DT Article
DE ;eDNA metabarcoding;sequence clustering;Operational Taxonomic Unit;diversity assessment;detectability
AB Environmental DNA (eDNA) has the potential to provide more comprehensive biodiversity assessments, particularly for vertebrates in species-rich regions. However, this method requires the completeness of a reference database (i.e. a list of DNA sequences attached to each species), which is not currently achieved for many taxa and ecosystems. As an alternative, a range of operational taxonomic units (OTUs) can be extracted from eDNA metabarcoding. However, the extent to which the diversity of OTUs provided by a limited eDNA sampling effort can predict regional species diversity is unknown. Here, by modelling OTU accumulation curves of eDNA seawater samples across the Coral Triangle, we obtained an asymptote reaching 1531 fish OTUs, while 1611 fish species are recorded in the region. We also accurately predict (R² = 0.92) the distribution of species richness among fish families from OTU-based asymptotes. Thus, the multi-model framework of OTU accumulation curves extends the use of eDNA metabarcoding in ecology, biogeography and conservation. 
PY 2020
PD JUN
SO Proceedings Of The Royal Society B-biological Sciences
SN 0962-8452
PU The Royal Society
VL 287
IS 1930
UT 000552605500001
DI 10.1098/rspb.2020.0248
ID 75232
ER

EF