FN Archimer Export Format
PT J
TI Role of RadA and DNA Polymerases in Recombination-Associated DNA Synthesis in Hyperthermophilic Archaea
BT 
AF Hogrel, Gaelle
   LU, Yang
   Alexandre, Nicolas
   Bossé, Audrey
   Dulermo, Remi
   Ishino, Sonoko
   Ishino, Yoshizumi
   Flament, Didier
AS 1:2,4;2:;3:2,4;4:1,2;5:1,2;6:3;7:3;8:1,2;
FF 1:;2:PDG-REM-EEP-LMEE;3:;4:PDG-REM-EEP-LMEE;5:PDG-REM-EEP-LMEE;6:;7:;8:PDG-REM-EEP-LMEE;
C1 Laboratoire de Microbiologie des Environnements Extrêmes, Ifremer, CNRS, Univ Brest, 29280 Plouzané, France
   LIA1211 MICROBSEA, Sino-French International Laboratory of Deep-Sea Microbiology, 29280 Xiamen-Plouzané, France
   Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, Fukuoka 819-0395, Japan
   Laboratoire de Microbiologie des Environnements Extrêmes, Ifremer, CNRS, Univ Brest, 29280 Plouzané, France
C2 IFREMER, FRANCE
   MICROBSEA, FRANCE
   UNIV KYUSHU, JAPAN
   UBO, FRANCE
SI BREST
SE PDG-REM-EEP-LMEE
UM BEEP-LM2E
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 4.694
TC 6
UR https://archimer.ifremer.fr/doc/00641/75274/75539.pdf
   https://archimer.ifremer.fr/doc/00641/75274/75540.pdf
LA English
DT Article
DE ;homologous recombination;Archaea;DNA polymerase;recombinase;DNA repair
AB Among the three domains of life, the process of homologous recombination (HR) plays a central role in the repair of double-strand DNA breaks and the restart of stalled replication forks. Curiously, main protein actors involved in the HR process appear to be essential for hyperthermophilic Archaea raising interesting questions about the role of HR in replication and repair strategies of those Archaea living in extreme conditions. One key actor of this process is the recombinase RadA, which allows the homologous strand search and provides a DNA substrate required for following DNA synthesis and restoring genetic information. DNA polymerase operation after the strand exchange step is unclear in Archaea. Working with Pyrococcus abyssi proteins, here we show that both DNA polymerases, family-B polymerase (PolB) and family-D polymerase (PolD), can take charge of processing the RadA-mediated recombination intermediates. Our results also indicate that PolD is far less efficient, as compared with PolB, to extend the invaded DNA at the displacement-loop (D-loop) substrate. These observations coincide with previous genetic analyses obtained on Thermococcus species showing that PolB is mainly involved in DNA repair without being essential probably because PolD could take over combined with additional partners. 
PY 2020
PD JUN
SO Biomolecules
SN 2218-273X
PU MDPI AG
VL 10
IS 7
UT 000559513400001
DI 10.3390/biom10071045
ID 75274
ER

EF