TY - JOUR T1 - Phytochemical profile, antioxidant and antibacterial activity of four Hypericum species from the UK A1 - Saddiqe,Zeb A1 - Naeem,Ismat A1 - Hellio,Claire A1 - Patel,Asmita V. A1 - Abbas,Ghulam AD - Department of Botany, Lahore College for Women University, Lahore, Pakistan AD - Department of Chemistry, Lahore College for Women University, Lahore, Pakistan AD - Univ Brest, Laboratoire des Sciences de l'Environnement MARin (LEMAR) CNRS, IRD, Ifremer F-29280, Plouzané, France AD - School of Pharmacy and Biomedical Sciences, University of Portsmouth, UK AD - Biological Sciences and Chemistry-Chemistry Section, College of Arts and Sciences, University of Nizwa, Oman UR - https://archimer.ifremer.fr/doc/00641/75309/ DO - 10.1016/j.sajb.2020.05.018 KW - Antibacterial activity KW - Antioxidants KW - Free radicals KW - Flavonoids KW - Hypericum species KW - Wound infections N2 - Treatment of skin wounds is an important domain in biomedical research since many pathogenic bacteria can invade the damaged tissues causing serious infections. Effective treatments are required under such conditions to inhibit microbial growth. Plants are traditionally used for the treatment of skin infections due to their antimicrobial potential. The antibacterial activity of different solvent extracts of four Hypericum species (H. androsaemum, H. ericoides, H. x moserianum and H. olympicum) traditionally acclaimed for their wound healing activity was examined in the present study against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Enterobacter aerogenes. In addition the content and types of flavonoids [High Performance Liquid Chromatography (HPLC) analysis], and antioxidant activity [1,1-diphenyl-2-picryIhydrazyl (DPPH) assay] were evaluated for all the species. The most prominent antibacterial activity was displayed by H. olympicum (MIC between 0.001 – 0.1 mg/mL) in particular ethyl acetate and n-butanol fractions which were found to be rich in phenolic and flavonoid contents. Strong antioxidant activity was observed for all the species and was associated with the more polar methanol, ethyl acetate and n-butanol extracts, with IC50 values ranging between 0.093 to 0.3 mg/mL. HPLC analysis of the extracts indicated the presence of different flavonoids in the plants and the highest content of selected flavonoids was determined for H. olympicum. The antibacterial activity of the selected Hypericum species shown in this study supports the traditional role of using these species for wound healing. Y1 - 2020/09 PB - Elsevier BV JF - South African Journal Of Botany SN - 0254-6299 VL - 133 SP - 45 EP - 53 ID - 75309 ER -