FN Archimer Export Format PT J TI Phytochemical profile, antioxidant and antibacterial activity of four Hypericum species from the UK BT AF Saddiqe, Zeb Naeem, Ismat Hellio, Claire Patel, Asmita V. Abbas, Ghulam AS 1:1;2:2;3:3;4:4;5:5; FF 1:;2:;3:;4:;5:; C1 Department of Botany, Lahore College for Women University, Lahore, Pakistan Department of Chemistry, Lahore College for Women University, Lahore, Pakistan Univ Brest, Laboratoire des Sciences de l'Environnement MARin (LEMAR) CNRS, IRD, Ifremer F-29280, Plouzané, France School of Pharmacy and Biomedical Sciences, University of Portsmouth, UK Biological Sciences and Chemistry-Chemistry Section, College of Arts and Sciences, University of Nizwa, Oman C2 UNIV LAHORE, PAKISTAN UNIV LAHORE, PAKISTAN UBO, FRANCE UNIV PORTSMOUTH, UK UNIV NIZWA, OMAN UM LEMAR IN WOS Cotutelle UMR copubli-europe copubli-int-hors-europe copubli-sud IF 2.315 TC 19 UR https://archimer.ifremer.fr/doc/00641/75309/75800.pdf LA English DT Article DE ;Antibacterial activity;Antioxidants;Free radicals;Flavonoids;Hypericum species;Wound infections AB Treatment of skin wounds is an important domain in biomedical research since many pathogenic bacteria can invade the damaged tissues causing serious infections. Effective treatments are required under such conditions to inhibit microbial growth. Plants are traditionally used for the treatment of skin infections due to their antimicrobial potential. The antibacterial activity of different solvent extracts of four Hypericum species (H. androsaemum, H. ericoides, H. x moserianum and H. olympicum) traditionally acclaimed for their wound healing activity was examined in the present study against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Enterobacter aerogenes. In addition the content and types of flavonoids [High Performance Liquid Chromatography (HPLC) analysis], and antioxidant activity [1,1-diphenyl-2-picryIhydrazyl (DPPH) assay] were evaluated for all the species. The most prominent antibacterial activity was displayed by H. olympicum (MIC between 0.001 – 0.1 mg/mL) in particular ethyl acetate and n-butanol fractions which were found to be rich in phenolic and flavonoid contents. Strong antioxidant activity was observed for all the species and was associated with the more polar methanol, ethyl acetate and n-butanol extracts, with IC50 values ranging between 0.093 to 0.3 mg/mL. HPLC analysis of the extracts indicated the presence of different flavonoids in the plants and the highest content of selected flavonoids was determined for H. olympicum. The antibacterial activity of the selected Hypericum species shown in this study supports the traditional role of using these species for wound healing. PY 2020 PD SEP SO South African Journal Of Botany SN 0254-6299 PU Elsevier BV VL 133 UT 000570949400007 BP 45 EP 53 DI 10.1016/j.sajb.2020.05.018 ID 75309 ER EF