FN Archimer Export Format PT J TI New Insight into Antimicrobial Compounds from Food and Marine-Sourced Carnobacterium Species through Phenotype and Genome Analyses BT AF Begrem, Simon Ivaniuk, Flora GIGOUT CHEVALIER, Frederique Kolypczuk, Laetitia Bonnetot, Sandrine Leroi, Francoise GROVEL, Olivier Delbarre-Ladrat, Christine Passerini, Delphine AS 1:1,2;2:2;3:2;4:2;5:2;6:2;7:1;8:2;9:2; FF 1:;2:PDG-RBE-BRM-LEMMMB;3:PDG-RBE-BRM-LEMMMB;4:PDG-RBE-BRM-LEMMMB;5:PDG-RBE-BRM-LEMMMB;6:PDG-RBE-BRM-LEMMMB;7:;8:PDG-RBE-BRM-LEMMMB;9:PDG-RBE-BRM-LEMMMB; C1 University of Nantes, 44035 Nantes CEDEX 1, France IFREMER, BRM, EM3B Laboratory, 44300 Nantes CEDEX 3, France C2 UNIV NANTES, FRANCE IFREMER, FRANCE SI NANTES SE PDG-RBE-BRM-LEMMMB IN WOS Ifremer UPR DOAJ copubli-france copubli-univ-france IF 4.167 TC 8 UR https://archimer.ifremer.fr/doc/00641/75335/75999.pdf https://archimer.ifremer.fr/doc/00641/75335/76000.zip LA English DT Article DE ;lactic acid bacteria;antimicrobial activity;Carnobacteriumspp;hydrogen peroxide;bacteriocin;RiPP;NRPS;terpene;natural product;genome mining AB Carnobacterium maltaromaticum and Carnobacterium divergens, isolated from food products, are lactic acid bacteria known to produce active and efficient bacteriocins. Other species, particularly those originating from marine sources, are less studied. The aim of the study is to select promising strains with antimicrobial potential by combining genomic and phenotypic approaches on large datasets comprising 12 Carnobacterium species. The biosynthetic gene cluster (BGCs) diversity of 39 publicly available Carnobacterium spp. genomes revealed 67 BGCs, distributed according to the species and ecological niches. From zero to six BGCs were predicted per strain and classified into four classes: terpene, NRPS (non-ribosomal peptide synthetase), NRPS-PKS (hybrid non-ribosomal peptide synthetase-polyketide synthase), RiPP (ribosomally synthesized and post-translationally modified peptide). In parallel, the antimicrobial activity of 260 strains from seafood products was evaluated. Among the 60% of active strains, three genomes were sequenced and submitted to a dereplication process. C. inhibens MIP2551 produced a high amountof H2O2, probably thanks to the presence of four oxidase-encoding genes. C. maltaromaticum EBP3019 and SF668 strains were highly efficient against Listeria monocytogenes. A new extracellular 16 kDa unmodified bacteriocin in the EBP3019 strain and five different bacteriocins in SF668 were highlighted. In this study, the overview of antimicrobial BGC and inhibitory activities of Carnobacterium spp. allowed the prediction of potential innovative natural products that could be relevant for biotechnological applications PY 2020 PD JUN SO Microorganisms SN 2076-2607 PU MDPI AG VL 8 IS 7 UT 000554816900001 DI 10.3390/microorganisms8071093 ID 75335 ER EF