FN Archimer Export Format
PT J
TI Taxonomy and toxicity of a bloom-forming Ostreopsis species (Dinophyceae, Gonyaulacales) in Tahiti island (South Pacific Ocean): one step further towards resolving the identity of O. siamensis.
BT 
AF Chomérat, Nicolas
   Bilien, Gwenael
   Viallon, Jérôme
   Hervé, Fabienne
   Réveillon, Damien
   Henry, Kévin
   Zubia, Mayalen
   Vieira, Christophe
   Ung, André
   Gatti, Clémence Mahana iti
   Roué, Mélanie
   DERRIEN, Amelie
   Amzil, Zouher
   Darius, Hélène Taiana
   Chinain, Mireille
AS 1:1;2:1;3:2;4:3;5:3;6:2;7:4;8:5;9:2;10:2;11:2;12:1,6;13:3;14:2;15:2;
FF 1:PDG-ODE-LITTORAL-LERBO;2:PDG-ODE-LITTORAL-LERBO;3:;4:PDG-ODE-DYNECO-PHYC;5:PDG-ODE-DYNECO-PHYC;6:;7:;8:;9:;10:;11:;12:PDG-ODE-LITTORAL-LERBO;13:PDG-ODE-DYNECO-PHYC;14:;15:;
C1 Ifremer, LER BO, Station of Marine Biology of Concarneau, Place de la Croix, F-29900 Concarneau, France
   Institut Louis Malardé, Laboratoire des Micro-algues toxiques, UMR 241-EIO, PO box 30, 98713 Papeete, Tahiti, French Polynesia
   Ifremer, Phycotoxins Laboratory, BP 21105, F-44311 Nantes Cedex 3, France
   Université de Polynésie Française, UMR 241-EIO, PO Box 6570, 98702 Faa'a, Tahiti, French Polynesia
   Kobe University Research Center for Inland Seas, Rokkodai, Kobe 657-8501, Japan
   Institut de Recherche pour le Développement (IRD), UMR 241-EIO, PO box 529, 98713 Papeete, Tahiti, French Polynesia
C2 IFREMER, FRANCE
   INST LOUIS MALARDE, FRANCE
   IFREMER, FRANCE
   UNIV POLYNESIE FRANCAISE, FRANCE
   UNIV KOBE, JAPAN
   IRD, FRANCE
SI CONCARNEAU
   NANTES
SE PDG-ODE-LITTORAL-LERBO
   PDG-ODE-DYNECO-PHYC
UM EIO
IN WOS Ifremer UPR
   WOS Cotutelle UMR
   copubli-france
   copubli-p187
   copubli-univ-france
   copubli-int-hors-europe
IF 4.273
TC 11
UR https://archimer.ifremer.fr/doc/00643/75504/76574.pdf
LA English
DT Article
DE ;Dinoflagellate;ITS-5.8S rDNA;LSU rDNA;Microscopy;Morphology;Ostreocin;Phylogeny
AB Among dinoflagellates responsible for benthic harmful algal blooms, the genus Ostreopsis primarily described from tropical areas has been increasingly reported from subtropical and temperate areas worldwide. Several species of this toxigenic genus produce analogs of palytoxin, thus representing a major threat to human and environmental health. The taxonomy of several species needs to be clarified as it was based mostly on morphological descriptions leading in some cases to ambiguous interpretations and misidentifications. The present study aims at reporting a benthic bloom that occurred in April 2019 in Tahiti island, French Polynesia. A complete taxonomic investigation of the blooming Ostreopsis species was realized using light, epifluorescence and field emission electron microscopy and phylogenetic analyses inferred from LSU rDNA and ITS–5.8S rDNA regions. Toxicity of a natural sample and strains isolated from the bloom was assessed using both neuroblastoma cell-based assay and LC-MS/MS analyses. Morphological observations showed that cells were round to oval, large, 58.0–82.5 µm deep (dorso-ventral length) and 45.7–61.2 µm wide. The cingulum was conspicuously undulated, forming a ‘V’ in ventral view. Thecal plates possessed large pores in depressions, with a collar rim. Detailed observation also revealed the presence of small thecal pores invisible in LM. Phylogenetic analyses were congruent and all sequences clustered within the genotype Ostreopsis sp. 6, in a subclade closely related to sequences from the Gulf of Thailand and Malaysia. No toxicity was found on the field sample but all the strains isolated from the bloom were found to be cytotoxic and produced ostreocin D, a lower amount of ostreocins A and B and putatively other compounds. Phylogenetic data demonstrate the presence of this species in the Gulf of Thailand, at the type locality of O. siamensis, and morphological data are congruent with the original description and support this identification. 
PY 2020
PD SEP
SO Harmful Algae
SN 1568-9883
PU Elsevier BV
VL 98
UT 000583196800005
DI 10.1016/j.hal.2020.101888
ID 75504
ER

EF