FN Archimer Export Format
PT J
TI Alterocin, an antibiofilm protein secreted by Pseudoalteromonas sp. 3J6
BT 
AF Jouault, Albane
   Gobet, Angelique
   Simon, Marjolaine
   Portier, Emilie
   Perennou, Morgan
   Corre, Erwan
   Gaillard, Fanny
   Vallenet, David
   Michel, Gurvan
   Fleury, Yannick
   Bazire, Alexis
   Dufour, Alain
AS 1:1;2:1,2,3;3:1;4:1;5:4;6:4;7:4;8:5;9:2;10:6;11:1;12:1;
FF 1:;2:PDG-RBE-MARBEC-LAAAS;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;
C1 Université de Bretagne-Sud, EA 3884, LBCM, IUEM, F-56100 Lorient, France
   Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models (LBI2M), Station Biologique de Roscoff (SBR), F-29680 Roscoff, France
   Univ Montpellier, CNRS, Ifremer, IRD, MARBEC, F-34203 Sète, France
   Sorbonne Université, CNRS, FR2424, Station Biologique de Roscoff (SBR), F-29680 Roscoff, France
   LABGeM, Génomique Métabolique, CEA, Genoscope, Institut François Jacob, CNRS, Université d'Evry, Université Paris-Saclay, F-91057 Evry, France
   Université de Brest, EA 3884, LBCM, IUEM, IUT Quimper, F-29000 Quimper, France
C2 UBS, FRANCE
   UNIV SORBONNE, FRANCE
   IFREMER, FRANCE
   UNIV SORBONNE, FRANCE
   CEA, FRANCE
   UBO, FRANCE
SI PALAVAS
SE PDG-RBE-MARBEC-LAAAS
UM MARBEC
IN WOS Ifremer UMR
   copubli-france
   copubli-univ-france
IF 4.792
TC 9
UR https://archimer.ifremer.fr/doc/00643/75517/76405.pdf
LA English
DT Article
DE ;antibiofilm protein;Pseudoalteromonas;Pseudomonas aeruginosa;biofilm
AB The aim was to identify and study the antibiofilm protein secreted by the marine bacterium Pseudoalteromonas sp. 3J6. The latter is active against marine and terrestrial bacteria, including Pseudomonas aeruginosa clinical strains forming different biofilm types. Several amino acid sequences were obtained from the partially purified antibiofilm protein, named alterocin. The Pseudoalteromonas sp. 3J6 genome was sequenced and a candidate alt gene was identified by comparing the genome-encoded proteins to the sequences from purified alterocin. Expressing the alt gene in another non-active Pseudoalteromonas sp. strain, 3J3, demonstrated that it is responsible for the antibiofilm activity. Alterocin is a 139-residue protein including a predicted 20-residue signal sequence, which would be cleaved off upon export by the general secretion system. No sequence homology was found between alterocin and proteins of known functions. The alt gene is not part of an operon and adjacent genes do not seem related to alterocin production, immunity or regulation, suggesting that these functions are not fulfilled by devoted proteins. During liquid growth, the alt mRNA level peaked during the stationary phase. A single promoter was experimentally identified and several inverted repeats could be binding sites for regulators. alt genes were found in about 30% of the Pseudoalteromonas genomes, and in only few instances of other marine bacteria of the Hahella and Paraglaciecola genera. Comparative genomics yielded the hypotheses that alt gene losses occurred within the Pseudoalteromonas genus. Overall, alterocin is a novel kind of antibiofilm protein of ecological and biotechnological interest. Importance Biofilms are microbial communities that develop on solid surfaces or interfaces, and are detrimental in a number of fields including for example food industry, aquaculture, and medicine. In the latter, antibiotics are insufficient to clear biofilm infections, leading to chronic infections such as in the case of infection by Pseudomonas aeruginosa of the lungs of cystic fibrosis patients. Antibiofilm molecules are thus urgently needed to be used in conjunction with conventional antibiotics, as well as in other fields of application, especially if they are environmentally friendly molecules. Here, we describe alterocin, a novel antibiofilm protein secreted by a marine bacterium belonging to the Pseudoalteromonas genus, and its gene. Alterocin homologs were found in about 30% of Pseudoalteromonas strains, indicating that this new family of antibiofilm proteins likely plays an important albeit non-essential function in the biology of these bacteria. This work opens up the possibility of a variety of applications. 
PY 2020
PD OCT
SO Applied And Environmental Microbiology
SN 0099-2240
PU American Society for Microbiology
VL 86
IS 20
UT 000582928300003
DI 10.1128/AEM.00893-20
ID 75517
ER

EF