FN Archimer Export Format
PT J
TI Nanopolystyrene beads affect motility and reproductive success of oyster spermatozoa ( Crassostrea gigas )
BT 
AF Tallec, Kevin
   Paul-Pont, Ika
   Boulais, Myrina
   Le Goïc, Nelly
   Gonzalez Fernandez, C.
   Le Grand, Fabienne
   Bideau, Antoine
   Quéré, Claudie
   Cassone, A.-L.
   Lambert, Christophe
   Soudant, Philippe
   Huvet, Arnaud
AS 1:1;2:2;3:1;4:2;5:2;6:2;7:2;8:1;9:2;10:2;11:2;12:1;
FF 1:PDG-RBE-PFOM-LPI;2:;3:PDG-RBE-PFOM-PI;4:;5:;6:;7:;8:PDG-RBE-PFOM-LPI;9:;10:;11:;12:PDG-RBE-PFOM-LPI;
C1 Univ Brest, Ifremer, CNRS, IRD, LEMAR, Plouzané, France
   Univ Brest, Ifremer, CNRS, IRD, LEMAR, Plouzané, France
C2 IFREMER, FRANCE
   UBO, FRANCE
SI BREST
SE PDG-RBE-PFOM-LPI
   PDG-RBE-PFOM-PI
UM LEMAR
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   copubli-france
   copubli-univ-france
IF 5.913
TC 24
UR https://archimer.ifremer.fr/doc/00643/75522/78210.pdf
LA English
DT Article
DE ;Nanoplastics;oyster;spermatozoa;motility;reproductive success
AB Oysters are keystone species that use external fertilization as a sexual mode. The gametes are planktonic and face a wide range of stressors, including plastic litter. Nanoplastics are of increasing concern because their size allows pronounced interactions with biological membranes, making them a potential hazard to marine life. In the present study, oyster spermatozoa were exposed for 1 h to various doses (from 0.1 to 25 µg mL−1) of 50-nm polystyrene beads with amine (50-NH2 beads) or carboxyl (50-COOH beads) functions. Microscopy revealed adhesion of particles to the spermatozoa membranes, but no translocation of either particle type into cells. Nevertheless, the 50-NH2 beads at 10 µg mL−1 induced a high spermiotoxicity, characterized by a decrease in the percentage of motile spermatozoa (−79%) and in the velocity (−62%) compared to control spermatozoa, with an overall drop in embryogenesis success (−59%). This major reproduction failure could be linked to a homeostasis disruption in exposed spermatozoa. The 50-COOH beads hampered spermatozoa motility only when administered at 25 µg mL−1 and caused a decrease in the percentage of motile spermatozoa (−66%) and in the velocity (−38%), but did not affect embryogenesis success. Microscopy analyses indicated these effects were probably due to physical blockages by microscale aggregates formed by the 50-COOH beads in seawater. This toxicological study emphasizes that oyster spermatozoa are a useful and sensitive model for (i) deciphering the fine interactions underpinning nanoplastic toxicity and (ii) evaluating adverse effects of plastic nanoparticles on marine biota while waiting for their concentration to be known in the environment. 
PY 2020
PD SEP
SO Nanotoxicology
SN 1743-5390
PU Informa UK Limited
VL 14
IS 8
UT 000560848100001
BP 1039
EP 1057
DI 10.1080/17435390.2020.1808104
ID 75522
ER

EF