FN Archimer Export Format
PT J
TI A method to assess algicidal activity of microalgal extracts coupling microalgae produced in stirred closed photobioreactor operating in continuous with pulse amplitude modulated (PAM) fluorometry
BT 
AF Menguy, Eva
   Dumontet, Vincent
   Coulombier, Noémie
   Meriot, Vincent
   LE DEAN, Loic
   Barthelemy, Vanille
   Jauffrais, Thierry
AS 1:1;2:2;3:3;4:3;5:1;6:1;7:1;
FF 1:PDG-RBE-LEADNC;2:;3:;4:;5:PDG-RBE-LEADNC;6:PDG-RBE-LEADNC;7:PDG-RBE-LEADNC;
C1 Ifremer, IRD, Univ Nouvelle‐Calédonie, Univ La Réunion, CNRS, UMR 9220 ENTROPIE, 101 Promenade Roger Laroque, 98897, Noumea, New Caledonia
   Université Paris Saclay, CNRS, UPR 2301, ICSN, 1 Avenue de la Terrasse, F-91198 Gif-sur-Yvette, France
   ADECAL Technopole, 1 bis rue Berthelot, 98846, Noumea, New Caledonia
C2 IFREMER, FRANCE
   UNIV PARIS SACLAY, FRANCE
   ADECAL TECHNOPOLE, FRANCE
SI NOUMEA
SE PDG-RBE-LEADNC
UM ENTROPIE
IN WOS Ifremer UMR
   DOAJ
   copubli-france
   copubli-univ-france
TC 3
UR https://archimer.ifremer.fr/doc/00643/75524/76400.pdf
LA English
DT Article
DE ;Bioassay;Cytoxicity;Microalgae;Quantum efficiency;Fv/Fm;Non photochemical quenching;NPQ;electron transport rate;Phytochemistry;Anti algal
AB We describe in the present study a quick and reliable method based on chlorophyll a fluorescence to assess putative algicidal effect of different microalgal extracts. We couple microalgal production under chemostat cultivation mode to continuously produce a given microalgae species (e.g. Dunaliella salina in this study) at a stable physiological state to ease comparison between extracts tested; with a non-destructive method based on chlorophyll a fluorescence. Pulse amplitude modulated (PAM) fluorometry was used to assess over time the effect of different microalgal crude extracts on the efficiency of the photosystem II (PSII) of a tested microalgae (Dunaliella salina). • Microalgal production at stationary phase in stirred closed photobioreactor (PBR) operating in continuous have stable photophysiological parameters, which is a prerequisite to compare the impact of different algicidal compounds. • The combination of both methods, allows to quickly assess the algicidal effect of diverse microalgal (crude) extracts on the PSII efficiency of a tested microalgae. • The method may be used to identify and isolate algicidal molecules affecting algal PSII using a bio-guided isolation protocol. 
PY 2020
SO Methodsx
SN 2215-0161
PU Elsevier BV
VL 7
UT 000607657300002
DI 10.1016/j.mex.2020.101037
ID 75524
ER

EF