FN Archimer Export Format PT J TI Microbial Communities of the Shallow-Water Hydrothermal Vent Near Naples, Italy, and Chemosynthetic Symbionts Associated With a Free-Living Marine Nematode BT AF Bellec, Laure Cambon-Bonavita, Marie-Anne Durand, Lucile Aube, Johanne Gayet, Nicolas Sandulli, Roberto Brandily, Christophe Zeppilli, Daniela AS 1:1,2,3;2:2;3:2;4:2;5:1;6:4;7:1;8:1; FF 1:;2:PDG-REM-EEP-LMEE;3:PDG-REM-EEP-LMEE;4:PDG-REM-EEP-LMEE;5:PDG-REM-EEP-LEP;6:;7:PDG-REM-EEP-LEP;8:PDG-REM-EEP-LEP; C1 Ifremer, Centre Brest, REM/EEP/LEP, ZI de la Pointe du Diable, CS10070, Plouzané, France Laboratoire de Microbiologie des Environnements Extrêmes, Ifremer, CNRS, Univ Brest, Plouzané, France EPOC, UMR 5805, University of Bordeaux, Arcachon, France Laboratory of Marine Ecology, Department of Science and Technology, University of Naples “Parthenope,” Naples, Italy C2 IFREMER, FRANCE IFREMER, FRANCE UNIV BORDEAUX, FRANCE UNIV NAPLES, ITALY SI BREST SE PDG-REM-EEP-LMEE PDG-REM-EEP-LEP UM BEEP-LM2E IN WOS Ifremer UPR WOS Ifremer UMR DOAJ copubli-france copubli-europe copubli-univ-france IF 3.941 TC 11 UR https://archimer.ifremer.fr/doc/00643/75544/76449.pdf https://archimer.ifremer.fr/doc/00643/75544/76450.docx https://archimer.ifremer.fr/doc/00643/75544/76451.docx https://archimer.ifremer.fr/doc/00643/75544/76452.docx https://archimer.ifremer.fr/doc/00643/75544/76453.docx https://archimer.ifremer.fr/doc/00643/75544/76454.xlsx https://archimer.ifremer.fr/doc/00643/75544/76455.xlsx https://archimer.ifremer.fr/doc/00643/75544/76456.xlsx https://archimer.ifremer.fr/doc/00643/75544/76457.xlsx https://archimer.ifremer.fr/doc/00643/75544/76458.zip LA English DT Article DE ;nematode;shallow-water hydrothermal vent;sulfur-oxidizing bacteria;iron cycle;Zetaproteobacteria AB Shallow-water hydrothermal vents are widespread, especially in the Mediterranean Sea, owing to the active volcanism of the area. Apart free microbial communities’ investigations, few biological studies have been leaded yet. Investigations of microbial communities associated with Nematoda, an ecologically important group in sediments, can help to improve our overall understanding of these ecosystems. We used a multidisciplinary-approach, based on microscopic observations (scanning electron microscopy: SEM and Fluorescence In Situ Hybridization: FISH) coupled with a molecular diversity analysis using metabarcoding, based on the 16S rRNA gene (V3-V4 region), to characterize the bacterial community of a free-living marine nematode and its environment, the shallow hydrothermal vent near Naples (Italy). Observations of living bacteria in the intestine (FISH), molecular and phylogenetic analyses showed that this species of nematode harbors its own bacterial community, distinct from the surrounding sediment and water. Metabarcoding results revealed the specific microbiomes of the sediment from three sites of this hydrothermal area to be composed mainly of sulfur oxidizing and reducing related bacteria. PY 2020 PD AUG SO Frontiers In Microbiology SN 1664-302X PU Frontiers Media SA VL 11 UT 000568403800001 DI 10.3389/fmicb.2020.02023 ID 75544 ER EF