Updated pigment composition of Tisochrysis lutea and purification of fucoxanthin using centrifugal partition chromatography coupled to flash chromatography for the chemosensitization of melanoma cells

Type Article
Date 2020-10
Language English
Author(s) Gonçalves De Oliveira-Júnior Raimundo1, Grougnet Raphaël2, Bodet Pierre-Edouard1, Bonnet Antoine1, Nicolau ElodieORCID3, Jebali Ahlem2, Rumin Judith1, Picot Laurent1
Affiliation(s) 1 : La Rochelle Université, LIENSs UMRi CNRS 7266, 17042 La Rochelle, France
2 : Equipe Produits Naturels, Analyse, Synthèse, UMR CNRS 8038, Faculté de Pharmacie, Université de Paris (ex-Université Paris Descartes), 75006 Paris, France
3 : Laboratoire BRM/PBA, IFREMER, 44311 Nantes, France
Source Algal Research-biomass Biofuels And Bioproducts (2211-9264) (Elsevier BV), 2020-10 , Vol. 51 , P. 102035 (13p.)
DOI 10.1016/j.algal.2020.102035
WOS© Times Cited 4
Keyword(s) Tisochrysis lutea, Carotenoids, Pigments, Centrifugal partition chromatography, Melanoma, Multidrug resistance
Abstract

Tisochrysis lutea (T. lutea, ex T. Isochrysis galbana or T-Iso) is a marine haptophyte that was first isolated from Tahiti seawater. Because of its high content in lipids, this tropical species is commonly used in aquaculture to feed fishes, crustaceans and molluscs larvae. It is also a rich source of fucoxanthin with a high potential for nutraceutical, cosmetic and pharmaceutical applications. The purpose of the present study was to detail the pigment composition of T. lutea and to develop an efficient process to recover highly purified fucoxanthin. Using ultra performant liquid chromatography coupled to diode arrays and high-resolution mass spectrometry detectors (UPLC-DAD-MS/MS), we demonstrated for the first time the presence of echinenone, 3-hydroxy-echinenone and chlorophyll c2-monogalactosyldiacylglycerol [18:4/14:0] in unstressed cultures of T. lutea. The chemotaxonomic relevance of this updated pigment composition was discussed in relation to the Haptophyta phylum. A two-step purification of fucoxanthin was then optimized using centrifugal partition chromatography coupled to flash chromatography. This process allowed the efficient isolation of fucoxanthin (purity > 99%), that was further assessed as a low-toxicity antineoplastic and chemosensitizing natural product in human chemoresistant melanoma cells. This carotenoid exerted an antiproliferative activity in A2058 melanoma cells and reversed in vitro their chemoresistance to dacarbazine, a DNA-alkylating agent clinically used for the treatment of metastatic melanoma.

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Gonçalves De Oliveira-Júnior Raimundo, Grougnet Raphaël, Bodet Pierre-Edouard, Bonnet Antoine, Nicolau Elodie, Jebali Ahlem, Rumin Judith, Picot Laurent (2020). Updated pigment composition of Tisochrysis lutea and purification of fucoxanthin using centrifugal partition chromatography coupled to flash chromatography for the chemosensitization of melanoma cells. Algal Research-biomass Biofuels And Bioproducts, 51, 102035 (13p.). Publisher's official version : https://doi.org/10.1016/j.algal.2020.102035 , Open Access version : https://archimer.ifremer.fr/doc/00644/75653/