FN Archimer Export Format
PT J
TI Updated pigment composition of Tisochrysis lutea and purification of fucoxanthin using centrifugal partition chromatography coupled to flash chromatography for the chemosensitization of melanoma cells
BT 
AF Gonçalves de Oliveira-Júnior, Raimundo
   Grougnet, Raphaël
   Bodet, Pierre-Edouard
   Bonnet, Antoine
   Nicolau, Elodie
   Jebali, Ahlem
   Rumin, Judith
   Picot, Laurent
AS 1:1;2:2;3:1;4:1;5:3;6:2;7:1;8:1;
FF 1:;2:;3:;4:;5:PDG-RBE-BRM-LPBA;6:;7:;8:;
C1 La Rochelle Université, LIENSs UMRi CNRS 7266, 17042 La Rochelle, France
   Equipe Produits Naturels, Analyse, Synthèse, UMR CNRS 8038, Faculté de Pharmacie, Université de Paris (ex-Université Paris Descartes), 75006 Paris, France
   Laboratoire BRM/PBA, IFREMER, 44311 Nantes, France
C2 UNIV LA ROCHELLE, FRANCE
   UNIV PARIS, FRANCE
   IFREMER, FRANCE
SI NANTES
SE PDG-RBE-BRM-LPBA
IN WOS Ifremer UPR
   copubli-france
   copubli-univ-france
IF 4.401
TC 21
UR https://archimer.ifremer.fr/doc/00644/75653/77271.pdf
LA English
DT Article
DE ;Tisochrysis lutea;Carotenoids;Pigments;Centrifugal partition chromatography;Melanoma;Multidrug resistance
AB Tisochrysis lutea (T. lutea, ex T. Isochrysis galbana or T-Iso) is a marine haptophyte that was first isolated from Tahiti seawater. Because of its high content in lipids, this tropical species is commonly used in aquaculture to feed fishes, crustaceans and molluscs larvae. It is also a rich source of fucoxanthin with a high potential for nutraceutical, cosmetic and pharmaceutical applications. The purpose of the present study was to detail the pigment composition of T. lutea and to develop an efficient process to recover highly purified fucoxanthin. Using ultra performant liquid chromatography coupled to diode arrays and high-resolution mass spectrometry detectors (UPLC-DAD-MS/MS), we demonstrated for the first time the presence of echinenone, 3-hydroxy-echinenone and chlorophyll c2-monogalactosyldiacylglycerol [18:4/14:0] in unstressed cultures of T. lutea. The chemotaxonomic relevance of this updated pigment composition was discussed in relation to the Haptophyta phylum. A two-step purification of fucoxanthin was then optimized using centrifugal partition chromatography coupled to flash chromatography. This process allowed the efficient isolation of fucoxanthin (purity > 99%), that was further assessed as a low-toxicity antineoplastic and chemosensitizing natural product in human chemoresistant melanoma cells. This carotenoid exerted an antiproliferative activity in A2058 melanoma cells and reversed in vitro their chemoresistance to dacarbazine, a DNA-alkylating agent clinically used for the treatment of metastatic melanoma. 
PY 2020
PD OCT
SO Algal Research-biomass Biofuels And Bioproducts
SN 2211-9264
PU Elsevier BV
VL 51
UT 000576964100012
DI 10.1016/j.algal.2020.102035
ID 75653
ER

EF