FN Archimer Export Format
PT J
TI Effects of Nitrogen Availability on the Antioxidant Activity and Carotenoid Content of the Microalgae Nephroselmis sp
BT 
AF Coulombier, Noémie
   Nicolau, Elodie
   Le Déan, Loic
   Barthelemy, Vanille
   Schreiber, Nathalie
   Brun, Pierre
   Lebouvier, Nicolas
   Jauffrais, Thierry
AS 1:1;2:2;3:3;4:3;5:2;6:3;7:4;8:3;
FF 1:;2:PDG-RBE-BRM-LPBA;3:PDG-RBE-LEADNC;4:PDG-RBE-LEADNC;5:PDG-RBE-BRM-LPBA;6:PDG-RBE-LEADNC;7:;8:PDG-RBE-LEADNC;
C1 ADECAL Technopole, 1 bis rue Berthelot, 98846 Noumea, New Caledonia
   Ifremer, RBE/BRM/LPBA, Rue de l’île d’Yeu, 44311 Nantes, France
   Ifremer, IRD, Univ Nouvelle-Calédonie, Univ La Réunion, UMR 9220 ENTROPIE, BP 32078, 98800 Nouméa, New Caledonia
   ISEA, EA7484, Université de la Nouvelle Calédonie, Campus de Nouville, 98851 Nouméa, New Caledonia
C2 ADECAL TECHNOPOLE, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
   UNIV NOUVELLE CALEDONIE, FRANCE
SI NANTES
   NOUMEA
   SAINT VINCENT
SE PDG-RBE-BRM-LPBA
   PDG-RBE-LEADNC
UM ENTROPIE
IN WOS Ifremer UPR
   WOS Ifremer UMR
   DOAJ
   copubli-france
   copubli-univ-france
IF 1.103
TC 23
UR https://archimer.ifremer.fr/doc/00645/75699/76604.pdf
   https://archimer.ifremer.fr/doc/00645/75699/76605.pdf
LA English
DT Article
DE ;lutein;natural products;nutrients;peroxyl radical;siphonaxanthin
AB Nephroselmis sp. was previously identified as a species of interest for its antioxidant properties owing to its high carotenoid content. In addition, nitrogen availability can impact biomass and specific metabolites’ production of microalgae. To optimize parameters of antioxidant production, Nephroselmis sp. was cultivated in batch and continuous culture conditions in stirred closed photobioreactors under different nitrogen conditions (N-repletion, N-limitation, and N-starvation). The aim was to determine the influence of nitrogen availability on the peroxyl radical scavenging activity (oxygen radical absorbance capacity (ORAC) assay) and carotenoid content of Nephroselmis sp. Pigment analysis revealed a specific and unusual photosynthetic system with siphonaxanthin-type light harvesting complexes found in primitive green algae, but also high lutein content and xanthophyll cycle pigments (i.e., violaxanthin, antheraxanthin, and zeaxanthin), as observed in most advanced chlorophytes. The results indicated that N-replete conditions enhance carotenoid biosynthesis, which would correspond to a higher antioxidant capacity measured in Nephroselmis sp. Indeed, peroxyl radical scavenging activity and total carotenoids were higher under N-replete conditions and decreased sharply under N-limitation or starvation conditions. Considering individual carotenoids, siphonaxanthin, neoxanthin, xanthophyll cycle pigments, and lycopene followed the same trend as total carotenoids, while β-carotene and lutein stayed stable regardless of the nitrogen availability. Carotenoid productivities were also higher under N-replete treatment. The peroxyl radical scavenging activity measured with ORAC assay (63.6 to 154.9 µmol TE g−1 DW) and the lutein content (5.22 to 7.97 mg g−1 DW) were within the upper ranges of values reported previously for other microalgae. Furthermore, contents of siphonaxanthin ere 6 to 20% higher than in previous identified sources (siphonous green algae). These results highlight the potential of Nephroselmis sp. as a source of natural antioxidant and as a pigment of interest. 
PY 2020
PD SEP
SO Marine Drugs
SN 1660-3397
PU MDPI AG
VL 18
IS 9
UT 000580318500001
DI 10.3390/md18090453
ID 75699
ER

EF