FN Archimer Export Format
PT J
TI A comparison of behavioral and reproductive parameters between wild-type, transgenic and mutant zebrafish: Could they all be considered the same “zebrafish” for reglementary assays on endocrine disruption?
BT 
AF De Oliveira, Julie
   Chadili, Edith
   Turies, Cyril
   Brion, François
   Cousin, Xavier
   Hinfray, Nathalie
AS 1:1;2:1;3:1;4:1;5:2,3;6:1;
FF 1:;2:;3:;4:;5:;6:;
C1 INERIS, Unité d'écotoxicologie in vitro et in vivo, UMR I-02 SEBIO, Verneuil-en-Halatte, France
   MARBEC Univ. Montpellier, CNRS, Ifremer, IRD, Palavas-les-Flots, France
   Univ. Paris-Saclay, AgroParisTech, INRAE, GABI, France
C2 INERIS, FRANCE
   INRAE, FRANCE
   INRAE, FRANCE
UM MARBEC
IN WOS Cotutelle UMR
   copubli-france
IF 4.52
TC 3
UR https://archimer.ifremer.fr/doc/00646/75799/76721.pdf
LA English
DT Article
DE ;Zebrafish;Casper;Transgenic;Behavior;Reproduction
AB Transgenic zebrafish models are efficiently used to study the effects of endocrine disrupting chemicals (EDC); thereby informing on their mechanisms of action. However, given the reported differences between zebrafish strains at the genetical, physiological and behavioral levels; care should be taken before using these transgenic models for EDC testing. In the present study, we undertook a set of experiments in different transgenic and/or mutant zebrafish lines of interest for EDC testing: casper, cyp19a1a-eGFP, cyp19a1a-eGFP-casper, cyp11c1-eGFP, cyp11c1-eGFP-casper. Some behavioral traits, and some biochemical and reproductive physiological endpoints commonly used in EDC testing were assessed and compared to those obtained in WT AB zebrafish to ensure that transgene insertion and/or mutations do not negatively modify basal reproductive physiology or behavior of the fish. Behavioral traits considered as anxiety and sociality have been monitored. Sociality was evaluated by monitoring the time spent near congeners in a shuttle box while anxiety was evaluated using the Novel tank diving test. No critical difference was observed between strains for either sociality or anxiety level. Concerning reproduction, no significant difference in the number of eggs laid per female, in the viability of eggs or in the female circulating VTG concentrations was noted between the 5 transgenic/mutants and the WT AB zebrafish studied. In summary, the transgene insertion and the mutations had no influence on the endpoints measured in basal conditions. These results were a prerequisite to the use of these transgenic/mutant models for EDC testing. Next step will be to determine the sensitivity of these biological models to chemical exposure to accurately validate their use in existing fish assays for EDC testing. 
PY 2021
PD JAN
SO Comparative Biochemistry And Physiology C-toxicology & Pharmacology
SN 1532-0456
PU Elsevier BV
VL 239
UT 000588051700018
DI 10.1016/j.cbpc.2020.108879
ID 75799
ER

EF