Exploring Antifouling Activity of Biosurfactants Producing Marine Bacteria Isolated from Gulf of California

Type Article
Date 2020-09
Language English
Author(s) Alemán-Vega Monserrat1, Sánchez-Lozano Ilse1, Hernández-Guerrero Claudia J.1, Hellio Claire2, Quintana Erika T.3
Affiliation(s) 1 : Instituto Politécnico Nacional, Centro Interdisciplinario de Ciencias Marinas, Av. Instituto Politécnico Nacional S/N. Col. Playa Palo de Santa Rita, 23096 La Paz, Baja California Sur, Mexico
2 : Univ Brest, CNRS, IRD, Ifremer, LEMAR, Institut Universitaire Européen de la Mer, F-29280 Plouzané, France
3 : Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prolongación de Carpio y Plan de Ayala s/n, Col. Santo Tomás, Alcaldía Miguel Hidalgo, C.P. 11340 Ciudad de Mexico, Mexico
Source International Journal Of Molecular Sciences (1422-0067) (MDPI AG), 2020-09 , Vol. 21 , N. 17 , P. 6068 (19p.)
DOI 10.3390/ijms21176068
WOS© Times Cited 15
Note This article belongs to the Section Biochemistry
Keyword(s) adhesion, biofilm, biosurfactants, environmentally friendly antifouling, field assays, Bacillus niabensis, Ralstoniasp

Biofouling causes major problems and economic losses to marine and shipping industries. In the search for new antifouling agents, marine bacteria with biosurfactants production capability can be an excellent option, due to the amphipathic surface-active characteristic that confers antimicrobial and antibiofilm activities. The aim of this study was to evaluate the antifouling activity of biosurfactants producing marine bacteria from the Gulf of California. The cell free culture supernatant (CFCS) of Bacillus niabensis (S-69), Ralstonia sp. (S-74) (isolated from marine sediment) and of B. niabensis (My-30) (bacteria associated to the sponge Mycale ramulosa) were screened for production of biosurfactants (using hemolysis and drop collapse test, oil displacement and emulsifying activity). The toxicity and antifouling activity were evaluated against biofoulers (bacteria forming biofilm and macrofoulers) both in laboratory and field assays. The results indicate that all bacteria were biosurfactant producers, but the higher capability was shown by B. niabensis (My-30) with high emulsifying properties (E24) of 71%. The CFCS showed moderate toxicity but were considered non-toxic against Artemia franciscana at low concentrations. In the antifouling assay, the CFCS of both strains of B. niabensis showed the best results for the reduction of the biofilm formation (up 50%) against all Gram-positive bacteria and most Gram-negative bacteria with low concentrations. In the field assay, the CFCS of B. niabensis (My-30) led to the reduction of 30% of biofouling compared to the control. The results indicate that the biosurfactant produced by B. niabensis (My-30) has promising antifouling activity.

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Alemán-Vega Monserrat, Sánchez-Lozano Ilse, Hernández-Guerrero Claudia J., Hellio Claire, Quintana Erika T. (2020). Exploring Antifouling Activity of Biosurfactants Producing Marine Bacteria Isolated from Gulf of California. International Journal Of Molecular Sciences, 21(17), 6068 (19p.). Publisher's official version : https://doi.org/10.3390/ijms21176068 , Open Access version : https://archimer.ifremer.fr/doc/00646/75804/