FN Archimer Export Format
PT J
TI A functional m 6 A‐RNA methylation pathway in the oyster Crassostrea gigas assumes epitranscriptomic regulation of lophotrochozoan development
BT 
AF Le Franc, Lorane
   Bernay, Benoit
   Petton, Bruno
   Since, Marc
   Favrel, Pascal
   Rivière, Guillaume
AS 1:1,2;2:3;3:4;4:5;5:1,2;6:1,2;
FF 1:;2:;3:PDG-RBE-PFOM-LPI;4:;5:;6:;
C1 UNICAEN CNRS BOREA Normandie Univ Caen, France
   Laboratoire Biologie des organismes et Ecosystèmes aquatiques (BOREA) Muséum d’Histoire naturelle CNRS IRD Sorbonne Université, Université de Caen NormandieUniversité des Antilles Caen ,France
   UNICAEN, ICOREPROTEOGEN Core Facility Caen SF ,France
   Ifremer, Laboratoire des Sciences de l’Environnement Marin UMR 6539 CNRS/UBO/IRD/Ifremer Centre Bretagne Normandie Univ Plouzané, France
   UNICAEN ,Comprehensive Cancer Center F. Baclesse, SF ICORE PRISMM Core Facility Normandie Univ Caen ,France
C2 UNIV CAEN, FRANCE
   MNHN, FRANCE
   UNIV CAEN, FRANCE
   IFREMER, FRANCE
   UNIV CAEN, FRANCE
SI ARGENTON
SE PDG-RBE-PFOM-LPI
UM LEMAR
IN WOS Ifremer UMR
   copubli-france
   copubli-univ-france
IF 5.622
TC 3
UR https://archimer.ifremer.fr/doc/00648/76026/84877.pdf
LA English
DT Article
DE ;development;epitranscriptomics;methylation;oyster;RNA
AB N6‐methyladenosine (m6A) is a prevalent epitranscriptomic mark in eukaryotic RNA, with crucial roles for mammalian and ecdysozoan development. Indeed, m6A‐RNA and the related protein machinery are important for splicing, translation, maternal‐to‐zygotic transition and cell differentiation. However, to date, the presence of an m6A‐RNA pathway remains unknown in more distant animals, questioning the evolution and significance of the epitranscriptomic regulation. Therefore, we investigated the m6A‐RNA pathway in the oyster Crassostrea gigas, a lophotrochozoan model whose development was demonstrated under strong epigenetic influence. Using mass spectrometry and dot blot assays, we demonstrated that m6A‐RNA is actually present in the oyster and displays variations throughout early oyster development, with the lowest levels at the end of cleavage. In parallel, by in silico analyses, we were able to characterize at the molecular level a complete and conserved putative m6A machinery. The expression levels of the identified putative m6A writers, erasers and readers were strongly regulated across oyster development. Finally, RNA pull‐down coupled to LC‐MS/MS allowed us to prove the actual presence of readers able to bind m6A‐RNA and exhibiting specific developmental patterns. Altogether, our results demonstrate the conservation of a complete m6A‐RNA pathway in the oyster and strongly suggest its implication in early developmental processes including MZT. This first demonstration and characterization of an epitranscriptomic regulation in a lophotrochozoan model, potentially involved in the embryogenesis, bring new insights into our understanding of developmental epigenetic processes and their evolution. 
PY 2021
PD MAR
SO Febs Journal
SN 1742-464X
PU Wiley
VL 288
IS 5
UT 000564384600001
BP 1696
EP 1711
DI 10.1111/febs.15500
ID 76026
ER

EF