FN Archimer Export Format
PT J
TI Genetic pathways underpinning hormonal stress responses in fish exposed to short- and long-term warm ocean temperatures
BT 
AF Goikoetxea, Alexander
   Sadoul, Bastien
   Blondeau Bidet, Eva
   Aerts, Johan
   Blanc, Marie-Odile
   Parrinello, Hugues
   Barrachina, Célia
   Pratlong, Marine
   Geffroy, Benjamin
AS 1:2;2:1;3:2;4:3;5:1;6:4;7:4;8:4;9:1;
FF 1:;2:PDG-RBE-MARBEC-LAAAS;3:;4:;5:PDG-RBE-MARBEC-LSEA;6:;7:;8:;9:PDG-RBE-MARBEC-LAAAS;
C1 MARBEC Univ Montpellier, CNRS, Ifremer, IRD, Palavas-Les-Flots, France
   MARBEC Univ Montpellier, CNRS, Ifremer, IRD, Montpellier, France
   Stress Physiology Research Group, Faculty of Sciences, Ghent University, Ostend, Belgium
   MGX, BCM, Univ Montpellier, CNRS, INSERM, Montpellier, France
C2 IFREMER, FRANCE
   CNRS, FRANCE
   UNIV GHENT, BELGIUM
   UNIV MONTPELLIER, FRANCE
SI PALAVAS
SE PDG-RBE-MARBEC-LAAAS
   PDG-RBE-MARBEC-LSEA
UM MARBEC
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-europe
   copubli-univ-france
IF 6.263
TC 35
UR https://archimer.ifremer.fr/doc/00651/76275/77311.pdf
LA English
DT Article
DE ;Cortisol;Glucocorticoid receptors;Transcriptomics;Scales;Commercial fish
AB Changes in ocean water temperature associated with global climate change are bound to enormously affect fish populations, with potential major economic consequences in the aquaculture and fisheries industries. A link between temperature fluctuations and changes in fish stress response is well established. In this study, we aimed to assess the effects of a short- (4 days) or a long-term (4 months) exposure to warm temperature in the stress physiology of European sea bass (Dicentrarchus labrax) larvae and juveniles. First, cortisol (i.e. the main stress hormone in fishes) analysis was used to confirm that a steady and short-term elevation of temperature acts as a physiological stressful event in these fish, and cortisol release is indeed above a metabolic increase linked to temperature. Moreover, our results verified that measurement of cortisol released into the water can be reliably employed as a non-invasive indicator of acute thermal stress in experimental conditions. Secondly, the different effects on the genetic cascade underlying the stress response between long-term low or high thermal treatments were evaluated at two larval development stages via candidate-gene and whole-transcriptome approaches. Interestingly, opposite expression for some key stress genes (nr3c1, nr3c2 and hsd11b2) were observed between developmental stages, highlighting the distinct adaptive mechanisms controlling the primary and secondary responses to a stressor. Surprising expression patterns for some understudied genes involved in the stress axis were also revealed, including crhr1, mc2r, mc5r, trh or trhr, which should be further explored. Finally, evaluation of cortisol content in scales was successfully used as a biomarker of chronic thermal stress, with 10x more cortisol in fish kept at 21 °C vs 16 °C after 4 months, supporting the gene expression results observed. The use of such a method as a proxy of long-term stress, unprecedented in the literature, holds a vast array of applications in further research, in particular, in the investigation of the impact of global warming on wild fish populations. 
PY 2021
PD JAN
SO Ecological Indicators
SN 1470-160X
PU Elsevier BV
VL 120
UT 000591880800008
DI 10.1016/j.ecolind.2020.106937
ID 76275
ER

EF