FN Archimer Export Format PT J TI State of art and best practices for fatty acid analysis in aquatic sciences BT AF Couturier, Lydie Michel, Loic Amaro, Teresa Budge, Suzanne M da Costa, Elisabete De Troch, Marleen Di Dato, Valeria Fink, Patrick Giraldo, Carolina Le Grand, Fabienne Loaiza, Iván Mathieu-Resuge, Margaux Nichols, Peter D Parrish, Christopher C Sardenne, Fany Vagner, Marie Pernet, Fabrice Soudant, Philippe Browman, Howard AS 1:1;2:2;3:3;4:4;5:5;6:6;7:7;8:8;9:9;10:1;11:6;12:10;13:11;14:12;15:1;16:1;17:1;18:1;19:; FF 1:;2:PDG-REM-EEP-LEP;3:;4:;5:;6:;7:;8:;9:PDG-RBE-HMMN-LRHBL;10:;11:;12:;13:;14:;15:;16:;17:PDG-RBE-PFOM-LPI;18:;19:; C1 Univ Brest, Ifremer, CNRS, IRD, LEMAR, Plouzané F-29280, France Ifremer, Centre de Bretagne, REM/EEP, Laboratoire Environnement Profond, Plouzané 29280, France Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto (U.Porto), Av. General Norton de Matos s/n, Matosinhos 4450-208, Portugal Department of Process Engineering and Applied Science, Dalhousie University, 5273 DaCosta Row, Halifax, NS B3H 4R2, Canada CESAM - Centre for Environmental and Marine Studies & LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Santiago University Campus, Aveiro 3810-193, Portugal Department of Biology, Marine Biology, Ghent University, Krijgslaan 281/S8, Ghent 9000, Belgium Marine Biotechnology Department, Stazione Zoologica Anton Dohrn Napoli, Villa Comunale, Napoli, Italy Helmholtz Centre for Environmental Research – UFZ, Department Aquatic Ecosystem Analysis and Management and Department River Ecology, Brückstrasse 3a, 30114 Magdeburg, Germany Ifremer - Centre Manche Mer du Nord, Département Ressources Biologiques et Environnement, Laboratoire des Ressources Halieutiques, 150 Quai Gambetta, BP 699, Boulogne-sur-Mer 62321, France WasserCluster Lunz – Inter-University Center for Aquatic Ecosystems Research, Lunz am See 3293, Austria CSIRO Oceans and Atmosphere, GPO Box 1538, Hobart, TAS 7000, Australia Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, Newfoundland A1C 5S7, Canada C2 UBO, FRANCE IFREMER, FRANCE UNIV PORTO, PORTUGAL UNIV DALHOUSIE, CANADA UNIV SANTIAGO, PORTUGAL UNIV GHENT, BELGIUM STAZ ZOOL ANTON DOHRN, ITALY UFZ, GERMANY IFREMER, FRANCE WASSERCLUSTER LUNZ, AUSTRIA CSIRO OCEANS AND ATMOSPHERE, AUSTRALIA UNIV MEM NEWFOUNDLAND, CANADA SI BREST BOULOGNE SE PDG-REM-EEP-LEP PDG-RBE-HMMN-LRHBL PDG-RBE-PFOM-LPI UM LEMAR IN WOS Ifremer UPR WOS Ifremer UMR WOS Cotutelle UMR copubli-france copubli-europe copubli-univ-france copubli-int-hors-europe IF 3.593 TC 30 UR https://archimer.ifremer.fr/doc/00651/76279/77252.pdf LA English DT Article DE ;aquatic nutrition;biomarkers;fatty acids;marine and freshwater lipids;methods;recommended practices AB Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA data. We provide recommendations for best practices in field situations and advocate for protocol standardization and interlaboratory calibration. PY 2020 PD DEC SO Ices Journal Of Marine Science SN 1054-3139 PU Oxford University Press (OUP) VL 77 IS 7-8 UT 000610522000001 BP 2375 EP 2395 DI 10.1093/icesjms/fsaa121 ID 76279 ER EF