FN Archimer Export Format PT J TI Molluscicidal and parasiticidal activities of Eryngium triquetrum essential oil on Schistosoma mansoni and its intermediate snail host Biomphalaria glabrata, a double impact BT AF de Carvalho Augusto, Ronaldo Merad, Nadjiya Rognon, Anne Gourbal, Benjamin Bertrand, Cédric Djabou, Nassim Duval, David AS 1:1,2;2:3;3:1,2;4:1,2;5:4,5,6;6:3;7:1,2; FF 1:;2:;3:;4:;5:;6:;7:; C1 University Perpignan Via Domitia, IHPE, UMR 5244, CNRS, IFREMER, Perpignan, France University Montpellier, IHPE, UMR 5244, CNRS, IFREMER, Montpellier, France Faculté des Sciences, Département de Chimie, Université de Tlemcen, Laboratoire COSNA, Tlemcen, Algeria EPHE-UPVD-CNRS, USR 3278 CRIOBE, Université de Perpignan, Perpignan, France Laboratoire d’Excellence «CORAIL», Université de Perpignan, Perpignan, France S.A.S. AkiNaO, Perpignan, France C2 UNIV PERPIGNAN, FRANCE UNIV MONTPELLIER, FRANCE UNIV TIEMCEN, ALGERIA UNIV PERPIGNAN, FRANCE UNIV PERPIGNAN, FRANCE S.A.S. AKINAO, FRANCE UM IHPE IN WOS Cotutelle UMR DOAJ copubli-france copubli-univ-france copubli-int-hors-europe copubli-sud IF 3.876 TC 16 UR https://archimer.ifremer.fr/doc/00652/76382/77391.pdf https://archimer.ifremer.fr/doc/00652/76382/77392.tif LA English DT Article DE ;Biomphalaria glabrata;Eryngium triquetrum;Molluscicide;Oil;Parasiticide;Schistosoma mansoni AB Background Freshwater snails are the intermediate hosts of a large variety of trematode flukes such as Schistosoma mansoni responsible for one of the most important parasitic diseases caused by helminths, affecting 67 million people worldwide. Recently, the WHO Global Vector Control Response 2017–2030 (GVCR) programme reinforced its message for safer molluscicides as part of required strategies to strengthen vector control worldwide. Here, we present the essential oil from Eryngium triquetrum as a powerful product with molluscicide and parasiticide effect against S. mansoni and the snail intermediate host Biomphalaria glabrata. Methods In the present study, we describe using several experimental approaches, the chemical composition of E. triquetrum essential oil extract and its biological effects against the snail B. glabrata and its parasite S. mansoni. Vector and the free-swimming larval stages of the parasite were exposed to different oil concentrations to determine the lethal concentration required to produce a mortality of 50% (LC50) and 90% (LC90). In addition, toxic activity of this essential oil was analyzed against embryos of B. glabrata snails by monitoring egg hatching and snail development. Also, short-time exposure to sublethal molluscicide concentrations on S. mansoni miracidia was performed to test a potential effect on parasite infectivity on snails. Mortality of miracidia and cercariae of S. mansoni is complete for 5, 1 and 0.5 ppm of oil extract after 1 and 4 h exposure. Results The major chemical component found in E. triquetrum oil determined by GC-FID and GC/MS analyses is an aliphatic polyacetylene molecule, the falcarinol with 86.9–93.1% of the total composition. The LC50 and LC90 values for uninfected snails were 0.61 and 1.02 ppm respectively for 24 h exposure. At 0.5 ppm, the essential oil was two times more toxic to parasitized snails with a mortality rate of 88.8 ± 4.8%. Moderate embryonic lethal effects were observed at the concentration of 1 ppm. Severe surface damage in miracidia was observed with a general loss of cilia that probably cause their immobility. Miracidia exposed 30 min to low concentration of plant extract (0.1 ppm) were less infective with 3.3% of prevalence compare to untreated with a prevalence of 44%. Conclusions Essential oil extracted from E. triquetrum and falcarinol must be considered as a promising product for the development of new interventions for schistosomiasis control and could proceed to be tested on Phase II according to the WHO requirements. PY 2020 PD SEP SO Parasites & Vectors SN 1756-3305 PU Springer Science and Business Media LLC VL 13 IS 1 UT 000574656300003 DI 10.1186/s13071-020-04367-w ID 76382 ER EF