Variations in isotope incorporation rates and trophic discrimination factors of carbon and nitrogen stable isotopes in scales from three European sea bass (Dicentrarchus labrax) populations

Type Article
Date 2020-12
Language English
Author(s) Rodde Charles1, 2, 3, 9, de Verdal Hugues1, 2, Lefebvre Sebastien5, Menniti Christophe6, Vandeputte MarcORCID7, Clota Frederic7, Allal FrancoisORCID4, McKenzie David9, Benzie John A.H.3, Nahon Sarah8
Affiliation(s) 1 : CIRAD, UMR ISEM, F-34398 Montpellier, France
2 : ISEM, Université de Montpellier, CNRS, EPHE, IRD, Montpellier, France
3 : Worldfish, Jalan Batu Maung, Bayan Lepas, 11960 Penang, Malaysia
4 : MARBEC, Université de Montpellier, CNRS, Ifremer, IRD, Palavas-les-Flots, France
5 : Université de Lille, CNRS, Université Littoral Côte d'Opale, UMR 8187, LOG Laboratoire d'Océanologie et Géosciences, station marine de Wiemreux F-59000, France
6 : Centre de Formation et de Recherche sur les Environnements Méditerranéens (CEFREM), UMR 5110, Université de Perpignan Via Domitia, 66860 Perpignan, France
7 : Université Paris-Saclay, INRAE, AgroParisTech, GABI, F-78350 Jouy-en-Josas, France
8 : INRAE, Université de Pau et des Pays de l'Adour, E2S UPPA, UMR 1419, Nutrition, Métabolisme, Aquaculture, Saint Pée sur Nivelle F-64310, France
9 : MARBEC, Université de Montpellier, CNRS, Ifremer, IRD, Palavas-les-Flots, France
Source Journal Of Experimental Marine Biology And Ecology (0022-0981) (Elsevier BV), 2020-12 , Vol. 533 , P. 151468 (10p.)
DOI 10.1016/j.jembe.2020.151468
WOS© Times Cited 2
Keyword(s) Fish, Metabolic pathway, Time-dependant model, Mixing model,non-lethal sampling
Abstract

Carbon (δ13C) and nitrogen (δ15N) stable isotope analyses are used in marine ecology to study trophic relationships and migrations of species since they reflect dietary sources consumed which may vary geographically. However, better estimations of isotope incorporation rates and trophic discrimination factors (TDF) under controlled conditions are required. Moreover, variability of isotope incorporation rates and TDF among and within populations has been poorly described, especially in fish scales, whereas the use of non-lethal method is becoming a standard. This study aimed to experimentally assess whether carbon and nitrogen isotope incorporation rates (λC and λN, respectively) and TDF of scales vary in the European sea bass (Dicentrarchus labrax) among (1) Atlantic, West Mediterranean and East Mediterranean populations, (2) sexes and (3) individuals. Fish were reared under controlled conditions and switched from a diet 1 to a diet 2 with different δ13C and δ15N values. Scales were sampled repeatedly on 16 fish within the three populations, from the day of diet change (day 0) to the end of the experiment (day 217). Isotope incorporation rates of scales and TDF were determined using a time-dependent model. Isotopic carbon and nitrogen half-lives (t50C and t50N) were similar among the three populations but males had significantly lower t50C and t50N than females (29 ± 2 and 35 ± 2 days vs. 53 ± 7 and 80 ± 11 days, respectively). Females had higher growth rates but lower catabolic rates than males. Variability of λC and λN was large within sexes: t50C ranged from 17 to 159 days and t50N ranged from 18 to 342 days among individuals. Thus, variability between sexes and among individuals must be considered to avoid misinterpretation in field-based studies. For the 48 fish, TDF were 4.91 ± 0.03 and 2.46 ± 0.06‰ for carbon and nitrogen, respectively, and similar between sexes and among populations. Besides, TDF varied among individuals from 2.95 to 5.59‰ and from 0.93 to 3.55‰ for carbon and nitrogen, respectively. Empirical mixing models were run to estimate how different TDF influenced estimation of the contributions of food sources to diet of their consumer. The output differed considerably when using TDF from fish literature or those estimated herein, which confirms that a tissue-specific TDF must be used to avoid misinterpretation in field-based studies. Individual variation in TDF did not, however, influence estimation of the contributions of food sources, confirming that scales are a valid tissue for non-lethal sampling.

Previous article in issue

Full Text
File Pages Size Access
10 1 MB Access on demand
Author's final draft 33 928 KB Open access
Top of the page

How to cite 

Rodde Charles, de Verdal Hugues, Lefebvre Sebastien, Menniti Christophe, Vandeputte Marc, Clota Frederic, Allal Francois, McKenzie David, Benzie John A.H., Nahon Sarah (2020). Variations in isotope incorporation rates and trophic discrimination factors of carbon and nitrogen stable isotopes in scales from three European sea bass (Dicentrarchus labrax) populations. Journal Of Experimental Marine Biology And Ecology, 533, 151468 (10p.). Publisher's official version : https://doi.org/10.1016/j.jembe.2020.151468 , Open Access version : https://archimer.ifremer.fr/doc/00652/76390/