FN Archimer Export Format
PT J
TI Variations in isotope incorporation rates and trophic discrimination factors of carbon and nitrogen stable isotopes in scales from three European sea bass (Dicentrarchus labrax) populations
BT 
AF Rodde, Charles
   de Verdal, Hugues
   Lefebvre, Sebastien
   Menniti, Christophe
   Vandeputte, Marc
   Clota, Frederic
   Allal, Francois
   McKenzie, David
   Benzie, John A.H.
   Nahon, Sarah
AS 1:1,2,3,9;2:1,2;3:5;4:6;5:7;6:7;7:4;8:9;9:3;10:8;
FF 1:;2:;3:;4:;5:;6:;7:PDG-RBE-MARBEC-LAAAS;8:;9:;10:;
C1 CIRAD, UMR ISEM, F-34398 Montpellier, France
   ISEM, Université de Montpellier, CNRS, EPHE, IRD, Montpellier, France
   Worldfish, Jalan Batu Maung, Bayan Lepas, 11960 Penang, Malaysia
   MARBEC, Université de Montpellier, CNRS, Ifremer, IRD, Palavas-les-Flots, France
   Université de Lille, CNRS, Université Littoral Côte d'Opale, UMR 8187, LOG Laboratoire d'Océanologie et Géosciences, station marine de Wiemreux F-59000, France
   Centre de Formation et de Recherche sur les Environnements Méditerranéens (CEFREM), UMR 5110, Université de Perpignan Via Domitia, 66860 Perpignan, France
   Université Paris-Saclay, INRAE, AgroParisTech, GABI, F-78350 Jouy-en-Josas, France
   INRAE, Université de Pau et des Pays de l'Adour, E2S UPPA, UMR 1419, Nutrition, Métabolisme, Aquaculture, Saint Pée sur Nivelle F-64310, France
   MARBEC, Université de Montpellier, CNRS, Ifremer, IRD, Palavas-les-Flots, France
C2 CIRAD, FRANCE
   UNIV MONTPELLIER, FRANCE
   WORLDFISH, MALAYSIA
   IFREMER, FRANCE
   UNIV LILLE, FRANCE
   UNIV PERPIGNAN, FRANCE
   UNIV PARIS SACLAY, FRANCE
   INRAE, FRANCE
   UNIV MONTPELLIER, FRANCE
SI PALAVAS
SE PDG-RBE-MARBEC-LAAAS
UM MARBEC
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   copubli-france
   copubli-p187
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 2.171
TC 6
UR https://archimer.ifremer.fr/doc/00652/76390/93520.pdf
LA English
DT Article
DE ;Fish;Metabolic pathway;Time-dependant model;Mixing model,non-lethal sampling
AB Carbon (δ13C) and nitrogen (δ15N) stable isotope analyses are used in marine ecology to study trophic relationships and migrations of species since they reflect dietary sources consumed which may vary geographically. However, better estimations of isotope incorporation rates and trophic discrimination factors (TDF) under controlled conditions are required. Moreover, variability of isotope incorporation rates and TDF among and within populations has been poorly described, especially in fish scales, whereas the use of non-lethal method is becoming a standard. This study aimed to experimentally assess whether carbon and nitrogen isotope incorporation rates (λC and λN, respectively) and TDF of scales vary in the European sea bass (Dicentrarchus labrax) among (1) Atlantic, West Mediterranean and East Mediterranean populations, (2) sexes and (3) individuals. Fish were reared under controlled conditions and switched from a diet 1 to a diet 2 with different δ13C and δ15N values. Scales were sampled repeatedly on 16 fish within the three populations, from the day of diet change (day 0) to the end of the experiment (day 217). Isotope incorporation rates of scales and TDF were determined using a time-dependent model. Isotopic carbon and nitrogen half-lives (t50C and t50N) were similar among the three populations but males had significantly lower t50C and t50N than females (29 ± 2 and 35 ± 2 days vs. 53 ± 7 and 80 ± 11 days, respectively). Females had higher growth rates but lower catabolic rates than males. Variability of λC and λN was large within sexes: t50C ranged from 17 to 159 days and t50N ranged from 18 to 342 days among individuals. Thus, variability between sexes and among individuals must be considered to avoid misinterpretation in field-based studies. For the 48 fish, TDF were 4.91 ± 0.03 and 2.46 ± 0.06‰ for carbon and nitrogen, respectively, and similar between sexes and among populations. Besides, TDF varied among individuals from 2.95 to 5.59‰ and from 0.93 to 3.55‰ for carbon and nitrogen, respectively. Empirical mixing models were run to estimate how different TDF influenced estimation of the contributions of food sources to diet of their consumer. The output differed considerably when using TDF from fish literature or those estimated herein, which confirms that a tissue-specific TDF must be used to avoid misinterpretation in field-based studies. Individual variation in TDF did not, however, influence estimation of the contributions of food sources, confirming that scales are a valid tissue for non-lethal sampling. Previous article in issue 
PY 2020
PD DEC
SO Journal Of Experimental Marine Biology And Ecology
SN 0022-0981
PU Elsevier BV
VL 533
UT 000588286500006
DI 10.1016/j.jembe.2020.151468
ID 76390
ER

EF