Developmental Methylome of the Medicinal Plant Catharanthus roseus Unravels the Tissue-Specific Control of the Monoterpene Indole Alkaloid Pathway by DNA Methylation

Type Article
Date 2020-09
Language English
Author(s) Dugé De Bernonville Thomas1, Maury Stéphane2, Delaunay Alain2, Daviaud Christian3, Chaparro Cristian4, Tost Jörg3, O’connor Sarah Ellen5, Courdavault Vincent1
Affiliation(s) 1 : Faculté des Sciences et Techniques, Université de Tours, EA2106 Biomolécules et Biotechnologies Végétales, F-37200 Tours, France
2 : INRA, EA1207 USC1328 Laboratoire de Biologie des Ligneux et des Grandes Cultures, Université d’Orléans, F-45067 Orléans, France
3 : Laboratoire Epigénétique et Environnement, LEE, Centre National de Recherche en Génomique Humaine, Institut de Biologie François Jacob, F-92265 Evry, France
4 : CNRS, IFREMER, UMR5244 Interactions Hôtes-Pathogènes-Environnments, Université de Montpellier, Université de Perpignan Via Domitia, F-66860 Perpignan, France
5 : Max Planck Institute for Chemical Ecology, Department of Natural Product Biosynthesis, 07745 Jena, Germany
Source International Journal Of Molecular Sciences (1422-0067) (MDPI AG), 2020-09 , Vol. 21 , N. 17 , P. 6028 (26p.)
DOI 10.3390/ijms21176028
Note This article belongs to the Section Molecular Plant Sciences
Keyword(s) alkaloids, DNA methylation, epigenetics, folivory, organs, plant specialized metabolism
Abstract

Catharanthus roseus produces a wide spectrum of monoterpene indole alkaloids (MIAs). MIA biosynthesis requires a tightly coordinated pathway involving more than 30 enzymatic steps that are spatio-temporally and environmentally regulated so that some MIAs specifically accumulate in restricted plant parts. The first regulatory layer involves a complex network of transcription factors from the basic Helix Loop Helix (bHLH) or AP2 families. In the present manuscript, we investigated whether an additional epigenetic layer could control the organ-, developmental- and environmental-specificity of MIA accumulation. We used Whole-Genome Bisulfite Sequencing (WGBS) together with RNA-seq to identify differentially methylated and expressed genes among nine samples reflecting different plant organs and experimental conditions. Tissue specific gene expression was associated with specific methylation signatures depending on cytosine contexts and gene parts. Some genes encoding key enzymatic steps from the MIA pathway were found to be simultaneously differentially expressed and methylated in agreement with the corresponding MIA accumulation. In addition, we found that transcription factors were strikingly concerned by DNA methylation variations. Altogether, our integrative analysis supports an epigenetic regulation of specialized metabolisms in plants and more likely targeting transcription factors which in turn may control the expression of enzyme-encoding genes. View Full-Text

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Dugé De Bernonville Thomas, Maury Stéphane, Delaunay Alain, Daviaud Christian, Chaparro Cristian, Tost Jörg, O’connor Sarah Ellen, Courdavault Vincent (2020). Developmental Methylome of the Medicinal Plant Catharanthus roseus Unravels the Tissue-Specific Control of the Monoterpene Indole Alkaloid Pathway by DNA Methylation. International Journal Of Molecular Sciences, 21(17), 6028 (26p.). Publisher's official version : https://doi.org/10.3390/ijms21176028 , Open Access version : https://archimer.ifremer.fr/doc/00652/76453/