FN Archimer Export Format
PT J
TI Developmental Methylome of the Medicinal Plant Catharanthus roseus Unravels the Tissue-Specific Control of the Monoterpene Indole Alkaloid Pathway by DNA Methylation
BT 
AF Dugé de Bernonville, Thomas
   Maury, Stéphane
   Delaunay, Alain
   Daviaud, Christian
   Chaparro, Cristian
   Tost, Jörg
   O’Connor, Sarah Ellen
   Courdavault, Vincent
AS 1:1;2:2;3:2;4:3;5:4;6:3;7:5;8:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;
C1 Faculté des Sciences et Techniques, Université de Tours, EA2106 Biomolécules et Biotechnologies Végétales, F-37200 Tours, France
   INRA, EA1207 USC1328 Laboratoire de Biologie des Ligneux et des Grandes Cultures, Université d’Orléans, F-45067 Orléans, France
   Laboratoire Epigénétique et Environnement, LEE, Centre National de Recherche en Génomique Humaine, Institut de Biologie François Jacob, F-92265 Evry, France
   CNRS, IFREMER, UMR5244 Interactions Hôtes-Pathogènes-Environnments, Université de Montpellier, Université de Perpignan Via Domitia, F-66860 Perpignan, France
   Max Planck Institute for Chemical Ecology, Department of Natural Product Biosynthesis, 07745 Jena, Germany
C2 UNIV TOURS, FRANCE
   INRA, FRANCE
   CNRGH, FRANCE
   CNRS, FRANCE
   MAX PLANCK INST CHEM ECOL, GERMANY
UM IHPE
IN WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-europe
   copubli-univ-france
IF 1.467
TC 11
UR https://archimer.ifremer.fr/doc/00652/76453/77543.pdf
   https://archimer.ifremer.fr/doc/00652/76453/77544.zip
LA English
DT Article
DE ;alkaloids;DNA methylation;epigenetics;folivory;organs;plant specialized metabolism
AB Catharanthus roseus produces a wide spectrum of monoterpene indole alkaloids (MIAs). MIA biosynthesis requires a tightly coordinated pathway involving more than 30 enzymatic steps that are spatio-temporally and environmentally regulated so that some MIAs specifically accumulate in restricted plant parts. The first regulatory layer involves a complex network of transcription factors from the basic Helix Loop Helix (bHLH) or AP2 families. In the present manuscript, we investigated whether an additional epigenetic layer could control the organ-, developmental- and environmental-specificity of MIA accumulation. We used Whole-Genome Bisulfite Sequencing (WGBS) together with RNA-seq to identify differentially methylated and expressed genes among nine samples reflecting different plant organs and experimental conditions. Tissue specific gene expression was associated with specific methylation signatures depending on cytosine contexts and gene parts. Some genes encoding key enzymatic steps from the MIA pathway were found to be simultaneously differentially expressed and methylated in agreement with the corresponding MIA accumulation. In addition, we found that transcription factors were strikingly concerned by DNA methylation variations. Altogether, our integrative analysis supports an epigenetic regulation of specialized metabolisms in plants and more likely targeting transcription factors which in turn may control the expression of enzyme-encoding genes. View Full-Text 
PY 2020
PD SEP
SO International Journal Of Molecular Sciences
SN 1422-0067
PU MDPI AG
VL 21
IS 17
UT 000570068300001
DI 10.3390/ijms21176028
ID 76453
ER

EF