FN Archimer Export Format
PT J
TI Comparing environmental DNA metabarcoding and underwater visual census to monitor tropical reef fishes
BT 
AF Polanco Fernández, Andrea
   Marques, Virginie
   Fopp, Fabian
   Juhel, Jean‐Baptiste
   Borrero‐Pérez, Giomar Helena
   Cheutin, Marie‐Charlotte
   Dejean, Tony
   González Corredor, Juan David
   Acosta‐Chaparro, Andrés
   Hocdé, Régis
   Eme, David
   Maire, Eva
   Spescha, Manuel
   Valentini, Alice
   Manel, Stéphanie
   Mouillot, David
   Albouy, Camille
   Pellissier, Loïc
AS 1:1;2:2,3;3:4,5;4:2;5:1;6:2;7:6;8:1;9:1;10:2;11:7;12:2,8;13:4,5;14:6;15:3;16:2;17:7;18:4,5;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:PDG-RBE-EMH;12:;13:;14:;15:;16:;17:PDG-RBE-EMH;18:;
C1 Programa de Biodiversidad y Ecosistemas Marinos Museo de Historia Natural Marina de Colombia (MHNMC) Instituto de Investigaciones Marinas y Costeras‐INVEMAR Santa Marta, Colombia
   MARBEC CNRS Ifremer IRD University of Montpellier Montpellier, France
   EPHE CNRS UM, UM3 IRD UMR5175 CEFE PSL Research University Montpellier, France
   Landscape Ecology Institute of Terrestrial Ecosystems Department of Environmental Systems Science ETH Zürich Zürich ,Switzerland
   Unit of Land Change Science Swiss Federal Institute for Forest, Snow and Landscape Research WSL Birmensdorf ,Switzerland
   SPYGEN Le Bourget‐du‐Lac, France
   Unité Ecologie et Modèles pour l'Halieutique EMH IFREMER Nantes ,France
   Lancaster Environment Centre Lancaster University Lancaster, UK
C2 MHNMC, COLOMBIA
   UNIV MONTPELLIER, FRANCE
   UNIV MONTPELLIER, FRANCE
   ETH ZURICH, SWITZERLAND
   SWISS FED RES INST WSL, SWITZERLAND
   SPYGEN, FRANCE
   IFREMER, FRANCE
   UNIV LANCASTER, UK
SI NANTES
SE PDG-RBE-EMH
UM MARBEC
IN DOAJ
TC 0
UR https://archimer.ifremer.fr/doc/00653/76543/77643.pdf
   https://archimer.ifremer.fr/doc/00653/76543/77644.docx
LA English
DT Article
DE ;biodiversity;biomonitoring;Caribbean Sea;environmental DNA;reef fishes;underwater visual census
AB Environmental DNA (eDNA) analysis is a revolutionary method to monitor marine biodiversity from animal DNA traces. Examining the capacity of eDNA to provide accurate biodiversity measures in species‐rich ecosystems such as coral reefs is a prerequisite for their application in long‐term monitoring. Here, we surveyed two Colombian tropical marine reefs, the island of Providencia and Gayraca Bay near Santa Marta, using eDNA and underwater visual census (UVC) methods. We collected a large quantity of surface water (30 L per filter) above the reefs and applied a metabarcoding protocol using three different primer sets targeting the 12S mitochondrial DNA, which are specific to the vertebrates Actinopterygii and Elasmobranchii. By assigning eDNA sequences to species using a public reference database, we detected the presence of 107 and 85 fish species, 106 and 92 genera, and 73 and 57 families in Providencia and Gayraca Bay, respectively. Of the species identified using eDNA, 32.7% (Providencia) and 18.8% (Gayraca) were also found in the UVCs. We further found congruence in genus and species richness and abundance between eDNA and UVC approaches in Providencia but not in Gayraca Bay. Mismatches between eDNA and UVC had a phylogenetic and ecological signal, with eDNA detecting a broader phylogenetic diversity and more effectively detecting smaller species, pelagic species and those in deeper habitats. Altogether, eDNA can be used for fast and broad biodiversity surveys and is applicable to species‐rich ecosystems in the tropics, but improved coverage of the reference database is required before this new method could serve as an effective complement to traditional census methods. 
PY 2021
PD JAN
SO Environmental DNA
SN 2637-4943
PU Wiley
VL 3
IS 1
BP 142
EP 156
DI 10.1002/edn3.140
ID 76543
ER

EF