TY - JOUR T1 - The first head-tailed virus, MFTV1, infecting hyperthermophilic methanogenic deep-sea archaea A1 - Thiroux,Sarah A1 - Dupont,Samuel A1 - Nesbø,Camilla L. A1 - Bienvenu,Nadège A1 - Krupovic,Mart A1 - L'Haridon,Stephane A1 - Marie,Dominique A1 - Forterre,Patrick A1 - Godfroy,Anne A1 - Geslin,Claire AD - Univ Brest, CNRS, IFREMER, Laboratoire de Microbiologie des Environnements Extrêmes F‐29280 Plouzané, France AD - Biozone University of Toronto Toronto Ontario M5S 3E5 ,Canada AD - Department of Biological Sciences University of Alberta Edmonton AB T6G2R3, 12 ,Canada AD - Institut Pasteur, Unité de Biologie Moléculaire du Gène chez les Extrêmophiles, Département de Microbiologie 75015 Paris, France AD - Sorbonne University, UPMC Univ Paris 06, INSU‐CNRS, UMR 7144, Station Biologique de Roscoff 29680 Roscoff, France AD - Université Paris‐Saclay, Institut de biologie intégrative de la cellule, Laboratoire de Biologie Moléculaire du Gène chez les Extremophiles (LBMGE), UMR8621/CNRS 91405 Orsay Cedex, France AD - Univ Brest, CNRS, IFREMER, Laboratoire de Microbiologie des Environnements Extrêmes F‐29280 Plouzané, France UR - https://doi.org/10.1111/1462-2920.15271 DO - 10.1111/1462-2920.15271 N2 - Deep‐sea hydrothermal vents are inhabited by complex communities of microbes and their viruses. Despite the importance of viruses in controlling the diversity, adaptation and evolution of their microbial hosts, to date, only eight bacterial and two archaeal viruses isolated from abyssal ecosystems have been described. Thus, our efforts focused on gaining new insights into viruses associated with deep‐sea autotrophic archaea. Here, we provide the first evidence of an infection of a hyperthermophilic methanogenic archaea by a head‐tailed virus, Methanocaldococcus fervens tailed virus 1 (MFTV1). MFTV1 has an isometric head of 50 nm in diameter and a 150 nm‐long non‐contractile tail. Virions are released continuously without causing a sudden drop in host growth. MFTV1 infects Methanocaldococcus species and is the first hyperthermophilic head‐tailed virus described thus far. The viral genome is a double‐stranded linear DNA of 31 kb. Interestingly, our results suggest potential strategies adopted by the plasmid pMEFER01, carried by M. fervens, to spread horizontally in hyperthermophilic methanogens. The data presented here opens a new window of understanding on how the abyssal mobilome interacts with hyperthermophilic marine archaea. Y1 - 2021/07 PB - Wiley JF - Environmental Microbiology SN - 1462-2912 VL - 23 IS - 7 SP - 3614 EP - 3626 ID - 76544 ER -