FN Archimer Export Format PT J TI The first head-tailed virus, MFTV1, infecting hyperthermophilic methanogenic deep-sea archaea BT AF Thiroux, Sarah Dupont, Samuel Nesbø, Camilla L. Bienvenu, Nadège Krupovic, Mart L'Haridon, Stephane Marie, Dominique Forterre, Patrick Godfroy, Anne Geslin, Claire AS 1:1;2:7;3:2,3;4:1;5:4;6:1;7:5;8:4,6;9:7;10:1; FF 1:;2:PDG-REM-EEP-LMEE;3:;4:;5:;6:;7:;8:;9:PDG-REM-EEP-LMEE;10:; C1 Univ Brest, CNRS, IFREMER, Laboratoire de Microbiologie des Environnements Extrêmes F‐29280 Plouzané, France Biozone University of Toronto Toronto Ontario M5S 3E5 ,Canada Department of Biological Sciences University of Alberta Edmonton AB T6G2R3, 12 ,Canada Institut Pasteur, Unité de Biologie Moléculaire du Gène chez les Extrêmophiles, Département de Microbiologie 75015 Paris, France Sorbonne University, UPMC Univ Paris 06, INSU‐CNRS, UMR 7144, Station Biologique de Roscoff 29680 Roscoff, France Université Paris‐Saclay, Institut de biologie intégrative de la cellule, Laboratoire de Biologie Moléculaire du Gène chez les Extremophiles (LBMGE), UMR8621/CNRS 91405 Orsay Cedex, France Univ Brest, CNRS, IFREMER, Laboratoire de Microbiologie des Environnements Extrêmes F‐29280 Plouzané, France C2 UBO, FRANCE UNIV TORONTO, CANADA UNIV ALBERTA, CANADA INST PASTEUR, FRANCE UNIV SORBONNE, FRANCE UNIV PARIS SACLAY, FRANCE IFREMER, FRANCE SI BREST SE PDG-REM-EEP-LMEE UM BEEP-LM2E IN WOS Ifremer UMR WOS Cotutelle UMR copubli-france copubli-univ-france copubli-int-hors-europe IF 5.476 TC 13 UR https://archimer.ifremer.fr/doc/00653/76544/77645.pdf LA English DT Article CR PHARE BO L'Atalante AB Deep‐sea hydrothermal vents are inhabited by complex communities of microbes and their viruses. Despite the importance of viruses in controlling the diversity, adaptation and evolution of their microbial hosts, to date, only eight bacterial and two archaeal viruses isolated from abyssal ecosystems have been described. Thus, our efforts focused on gaining new insights into viruses associated with deep‐sea autotrophic archaea. Here, we provide the first evidence of an infection of a hyperthermophilic methanogenic archaea by a head‐tailed virus, Methanocaldococcus fervens tailed virus 1 (MFTV1). MFTV1 has an isometric head of 50 nm in diameter and a 150 nm‐long non‐contractile tail. Virions are released continuously without causing a sudden drop in host growth. MFTV1 infects Methanocaldococcus species and is the first hyperthermophilic head‐tailed virus described thus far. The viral genome is a double‐stranded linear DNA of 31 kb. Interestingly, our results suggest potential strategies adopted by the plasmid pMEFER01, carried by M. fervens, to spread horizontally in hyperthermophilic methanogens. The data presented here opens a new window of understanding on how the abyssal mobilome interacts with hyperthermophilic marine archaea. PY 2021 PD JUN SO Environmental Microbiology SN 1462-2912 PU Wiley VL 23 IS 7 UT 000579783800001 BP 3614 EP 3626 DI 10.1111/1462-2920.15271 ID 76544 ER EF