FN Archimer Export Format
PT CHAP
TI Measuring Histone Modifications in the Human Parasite Schistosoma mansoni.
BT imson D.J. (eds) Schistosoma mansoni. Methods in Molecular Biology, vol 2151. Print ISBN 978-1-0716-0634-6 Online ISBN 978-1-0716-0635-3. Chap.9, pp.93-107
AF De Carvalho Augusto, Ronaldo
   Roquis, David
   Al Picard, Marion
   Chaparro, Christian
   Cosseau, Celine
   Grunau, Christoph
AS 1:1,2;2:3;3:4;4:1;5:1;6:1;
FF 1:;2:;3:;4:;5:;6:;
C1 IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia Perpignan , France
   LBMC, Laboratoire de Biologie et Modélisation de la Cellule Univ Lyon, ENS de Lyon Lyon, France
   Amélioration des Grandes Cultures et Ressources Génétiques, Agroscope Changins, Switzerland
   Centre National de la Recherche Scientifique (CNRS), Laboratory MIVEGEC (CNRS IRD Uni Montpellier) Montpellier, France
C2 UNIV MONTPELLIER, FRANCE
   ENS LYON, FRANCE
   AGROSCOPE, SWITZERLAND
   CNRS, FRANCE
UM IHPE
UR https://archimer.ifremer.fr/doc/00653/76553/77671.pdf
LA English
DT Book section
DE ;Native chromatin immunoprecipitation;ChIPmentation;Histone modifications;Schistosomiasis;Trematode;Development
AB DNA-binding proteins play critical roles in many major processes such as development and sexual biology of Schistosoma mansoni and are important for the pathogenesis of schistosomiasis. Chromatin immunoprecipitation (ChIP) experiments followed by sequencing (ChIP-seq) are useful to characterize the association of genomic regions with posttranslational chemical modifications of histone proteins. Challenges in the standard ChIP protocol have motivated recent enhancements in this approach, such as reducing the number of cells required and increasing the resolution. In this chapter, we describe the latest advances made by our group in the ChIP methods to improve the standard ChIP protocol to reduce the number of input cells required and to increase the resolution and robustness of ChIP in S. mansoni. 
PY 2020
DI 10.1007/978-1-0716-0635-3_9
ID 76553
ER

EF