FN Archimer Export Format
PT J
TI Cobalamin Scarcity Modifies Carbon Allocation and Impairs DMSP Production Through Methionine Metabolism in the Haptophyte Microalgae Tisochrysis lutea
BT 
AF Nef, Charlotte
   Henry, Céline
   Nicolau, Elodie
   Bérard, Jean-Baptiste
   Hervé, Fabienne
   Caruana, Amandine
   Kaas, Raymond
   Mairet, Francis
   Garnier, Matthieu
AS 1:1;2:2;3:1;4:1;5:3;6:3;7:1;8:1;9:1;
FF 1:PDG-RBE-BRM-LPBA;2:;3:PDG-RBE-BRM-LPBA;4:PDG-RBE-BRM-LPBA;5:PDG-ODE-DYNECO-PHYC;6:PDG-ODE-DYNECO-PHYC;7:PDG-RBE-BRM-LPBA;8:PDG-RBE-BRM-LPBA;9:PDG-RBE-BRM-LPBA;
C1 IFREMER, Physiology and Biotechnology of Algae Laboratory, Nantes, France
   PAPPSO, Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France
   IFREMER, Phycotoxins Laboratory, Nantes, France
C2 IFREMER, FRANCE
   INRA, FRANCE
   IFREMER, FRANCE
SI NANTES
SE PDG-RBE-BRM-LPBA
   PDG-ODE-DYNECO-PHYC
IN WOS Ifremer UPR
   DOAJ
   copubli-france
   copubli-p187
IF 5.247
TC 3
UR https://archimer.ifremer.fr/doc/00654/76575/77711.pdf
   https://archimer.ifremer.fr/doc/00654/76575/77712.docx
   https://archimer.ifremer.fr/doc/00654/76575/77713.xlsx
LA English
DT Article
DE ;cobalamin;vitamin B-12;haptophyte microalgae;Tisochrysis lutea;DMSP;chemostat;comparative proteomics;metabolism
AB Cobalamin (vitamin B12) is a cobalt-containing enzymatic cofactor involved in methionine synthesis. Provided only by select bacteria and archaea in marine systems, this vitamin is known to limit primary production in different oceanic areas. Understanding the consequences of cobalamin limitation on phytoplankton physiology is of great interest, notably for cobalamin-dependent haptophytes that significantly contribute to oceanic carbon fixation and sulfur cycle through dimethyl sulfonio propionate (DMSP) synthesis. Here, the effect of cobalamin limitation was compared to nitrogen limitation on the model haptophyte Tisochrysis lutea grown in chemostats, combining comparative proteomics with the analysis of major macromolecules and specific osmolytes. Our results highlight the interconnection of carbon and DMSP metabolisms through the cobalamin-dependent methionine synthesis by showing that cobalamin scarcity impacts the mechanisms of carbon allocation and reduces DMSP quota. Conversely, proline production seemed to anticorrelate with cobalamin availability. In a boarder context, analysis of transcriptomes or genomes of main DMSP producers from different phytoplankton lineages suggests that most of them are cobalamin-dependent, which means that prokaryotic cobalamin synthesis exerts an important control on phytoplankton DMSP production in some regions of the world ocean. 
PY 2020
PD OCT
SO Frontiers In Marine Science
SN 2296-7745
PU Frontiers Media SA
VL 7
UT 000579826300001
DI 10.3389/fmars.2020.569560
ID 76575
ER

EF